Comparison of Methodologies for Detecting Trypanosoma Cruzi Parasites by Microscopic Observation of Microhematocrit Capillary Tubes

Overview

Journal Rev Soc Bras Med Trop

Specialty Tropical Medicine

Date 2019 Jul 5

PMID 31271620

Citations 6

Authors

Marina Vera-Ku

Guillermo Meza-Gonzalez

Yves Carlier

Carine Truyens

Rubi Gamboa-Leon

Affiliations

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Abstract

Introduction: The microscopic examination of microhematocrit tubes (mHCT) has been proposed as the gold standard for acute and congenital Chagas disease diagnosis. We compared different mHCT methodologies detecting T. cruzi parasites in the blood.

Methods: The rotating method, water mount, and immersion oil methods were compared for their suitability, sensitivity, and specificity.

Results: The rotating method was easier, faster, and more sensitive than the others with 100% specificity.

Conclusions: The rotating method is feasible for laboratory technicians with standard training in microscopic techniques and is recommended for the diagnosis of acute Chagas disease in primary health care facilities.

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Lozano N, Prescilla-Ledezma A, Calabuig E, Trelis M, Arce J, Lopez Hontangas J PLoS Negl Trop Dis. 2024; 18(11):e0012356.

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Experimental Infection and Chagas Disease-A Word of Caution.

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