A Di-iron Protein Recruited As an Fe[II] and Oxygen Sensor for Bacterial Chemotaxis Functions by Stabilizing an Iron-peroxy Species

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2019 Jul 5

PMID 31270241

Citations 11

Authors

Alise R Muok

Yijie Deng

Vadim M Gumerov

Jenna E Chong

Jennifer R DeRosa

Kurni Kurniyati

Rachael E Coleman

Kyle M Lancaster

Chunhao Li

Igor B Zhulin

Brian R Crane

Affiliations

Soon will be listed here.

Abstract

Many bacteria contain cytoplasmic chemoreceptors that lack sensor domains. Here, we demonstrate that such cytoplasmic receptors found in 8 different bacterial and archaeal phyla genetically couple to metalloproteins related to β-lactamases and nitric oxide reductases. We show that this oxygen-binding di-iron protein (ODP) acts as a sensor for chemotactic responses to both iron and oxygen in the human pathogen (). The ODP di-iron site binds oxygen at high affinity to reversibly form an unusually stable μ-peroxo adduct. Crystal structures of ODP from and the thermophile () in the Fe[III]-O, Zn[II], and apo states display differences in subunit association, conformation, and metal coordination that indicate potential mechanisms for sensing. In reconstituted systems, iron-peroxo ODP destabilizes the phosphorylated form of the receptor-coupled histidine kinase CheA, thereby providing a biochemical link between oxygen sensing and chemotaxis in diverse prokaryotes, including anaerobes of ancient origin.

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