Solution NMR Backbone Assignment Reveals Interaction-free Tumbling of Human Lineage-specific Olduvai Protein Domains

Overview

Journal Biomol NMR Assign

Publisher Springer

Specialties Molecular Biology
Nuclear Medicine

Date 2019 Jul 3

PMID 31264103

Citations 4

Authors

Aaron Issaian

Lauren Schmitt

Alexandra Born

Parker J Nichols

James Sikela

Kirk Hansen

Beat Vogeli

Morkos A Henen

Affiliations

Soon will be listed here.

Abstract

Olduvai protein domains, encoded primarily by NBPF genes, have been linked to both human brain evolution and cognitive diseases such as autism and schizophrenia. There are six primary domains that comprise the Olduvai family: three conserved domains (CON1-3) and three human lineage-specific domains (HLS1-3), which typically occur as a triplet (HLS1, HLS2 and HLS3). Herein, we present the solution NMR assignment of the backbone chemical shifts of the separate HLS1, 2 and 3 domains of NBPF15. Our data suggest that there is no change in the structure of the separate domains when compared to the full-length triplet (HLS1-HLS2-HLS3). We also demonstrate that there is no direct interaction between the three domains.

Citing Articles

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PMID: 39766865 PMC: 11675761. DOI: 10.3390/genes15121598.

Olduvai domain expression downregulates mitochondrial pathways: implications for human brain evolution and neoteny.

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Proteolytic activation of human-specific Olduvai domains by the furin protease.

Pacheco A, Issaian A, Davis J, Anderson N, Nemkov T, Paukovich N Int J Biol Macromol. 2022; 234:123041.

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Solution NMR backbone assignments of disordered Olduvai protein domain CON1 employing Hα-detected experiments.

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