Antioxidant Activity, α-glucosidase Inhibition and Phytochemical Profiling of Leaf Extracts

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2019 Jul 2

PMID 31259094

Citations 12

Authors

James William

Peter John

Muhammad Waseem Mumtaz

Ayoub Rashid Ch

Ahmad Adnan

Hamid Mukhtar

Shahzad Sharif

Syed Ali Raza

Muhammad Tayyab Akhtar

Affiliations

Soon will be listed here.

Abstract

Background: Diabetes mellitus type II (DMT-2) is a widely spread metabolic disorder both in developed and developing countries. The role of oxidative stress is well established in DMT-2 pathogenesis. The synthetic drugs for DMT-2 are associated with serious side complications. Antioxidant and α-glucosidase inhibitory actions of phytochemicals from various plant species are considered as an alternative to synthetic drugs for DMT-2 management. The present study aimed to evaluate the antioxidant activity, α-glucosidase inhibitory potential and phytochemical profiling of .

Methods: The total phenolic and flavonoid contents, in vitro antioxidant activity (α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging and phosphomolybdenum method) and α-glucosidase inhibition of ultrasonicated hydroethanolic leaf extracts were determined spectrophotometrically. The results of DPPH assay and α-glucosidase inhibition were reported in terms of IC value. The phytochemical profiling was accomplished by UHPLC-Q-TOF/MS/MS technique.

Results And Discussion: Findings leaped 60% ethanolic extract as rich fraction regarding total phenolic and flavonoid contents. The 60% ethanolic fraction was a promising source of natural antioxidants and α-glucosidase inhibitory agents as indicated by anti-radical and enzyme inibitory activities. Kaempferol, rutin, hesperetin 5-O-glucoside, kaempferol-coumaroyl-glucoside, luteolin 3-glucoside, Isorhamnetin-3-O-rutinoside, trimethoxyflavone derivatives and citric acid were identified by UHPLC-Q-TOF-MS/MS. These compounds were believed to be responsible for the strong antioxidant and enzyme inhibitory activity of plant extracts. The extensive metabolite profiling of was carried out the first time as never reported previously. The might be an appropriate choice to manage diabetes mellitus in an alternate way. The findings may be further exploited extensively for toxicity evaluation to proceed with functional food development having antidiabetic attributes.

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