Characteristics of Olfactory Ensheathing Cells and Microarray Analysis in Tupaia belangeri (Wagner, 1841)

Overview

Journal Mol Med Rep

Specialty Molecular Biology

Date 2019 Jul 2

PMID 31257532

Citations 1

Authors

Na Lin

Xiu-Juan Dong

Ting-Yong Wang

Wen-Ji He

Jing Wei

Hai-Ying Wu

Ting-Hua Wang

Affiliations

Soon will be listed here.

Abstract

Tree shrews are most closely related to the primates and so possess a number of advantages in experimental studies; they have been used as an animal model in bacterial and virus infection, cancer, endocrine system disease, and certain nervous system diseases. Their olfactory ensheathing cells (OECs) are able to release several cytokines to promote neuronal survival, regeneration and remyelination. The present study used western blot analysis to identify antibody specificity in protein extracts from whole tree shrew brains to identify the specificity of p75 nerve growth factor receptor (NGFR) derived from rabbits (75 kDa). OECs were cultured and isolated, then stained and identified using the antibodies for p75NGFR. To investigate the capacity of OECs to express cytokines and growth factors, microarray technology was used, and the analysis revealed that OECs were able to express 9,821 genes. Of these genes, 44 genes were from the neurotrophic factor family, which may indicate their potential in transplantation in vivo. The present study considered the function of OECs as revealed by other studies, and may contribute to future research.

Citing Articles

Gene and protein expression profiles of olfactory ensheathing cells from olfactory bulb olfactory mucosa.

Lan Y, Yang P, Zeng Z, Yadav N, Zhang L, Wang L Neural Regen Res. 2021; 17(2):440-449.

PMID: 34269221 PMC: 8463967. DOI: 10.4103/1673-5374.317986.

References

Li S, Meng Q, Zhang L . Experimental therapy of a platelet-activating factor antagonist (ginkgolide B) on photochemically induced thrombotic cerebral ischaemia in tree shrews. Clin Exp Pharmacol Physiol. 1999; 26(10):824-5. DOI: 10.1046/j.1440-1681.1999.03146.x. View

Copelman C, Cuzner M, Groome N, Diemel L . Temporal analysis of growth factor mRNA expression in myelinating rat brain aggregate cultures: increments in CNTF, FGF-2, IGF-I, and PDGF-AA mRNA are induced by antibody-mediated demyelination. Glia. 2000; 30(4):342-51. DOI: 10.1002/(sici)1098-1136(200006)30:4<342::aid-glia30>3.0.co;2-8. View

Cannella B, Pitt D, Capello E, Raine C . Insulin-like growth factor-1 fails to enhance central nervous system myelin repair during autoimmune demyelination. Am J Pathol. 2000; 157(3):933-43. PMC: 1885703. DOI: 10.1016/S0002-9440(10)64606-8. View

Diemel L, Jackson S, Cuzner M . Role for TGF-beta1, FGF-2 and PDGF-AA in a myelination of CNS aggregate cultures enriched with macrophages. J Neurosci Res. 2003; 74(6):858-67. DOI: 10.1002/jnr.10837. View

Keuker J, de Biurrun G, Luiten P, Fuchs E . Preservation of hippocampal neuron numbers and hippocampal subfield volumes in behaviorally characterized aged tree shrews. J Comp Neurol. 2003; 468(4):509-17. DOI: 10.1002/cne.10996. View

Woodhouse A, Vincent A, Kozel M, Chung R, Waite P, Vickers J . Spinal cord tissue affects ensheathing cell proliferation and apoptosis. Neuroreport. 2005; 16(7):737-40. DOI: 10.1097/00001756-200505120-00016. View

van der Hart M, de Biurrun G, Czeh B, Rupniak N, den Boer J, Fuchs E . Chronic psychosocial stress in tree shrews: effect of the substance P (NK1 receptor) antagonist L-760735 and clomipramine on endocrine and behavioral parameters. Psychopharmacology (Berl). 2005; 181(2):207-16. DOI: 10.1007/s00213-005-2260-0. View

Czeh B, Simon M, Schmelting B, Hiemke C, Fuchs E . Astroglial plasticity in the hippocampus is affected by chronic psychosocial stress and concomitant fluoxetine treatment. Neuropsychopharmacology. 2006; 31(8):1616-26. DOI: 10.1038/sj.npp.1300982. View

Pastrana E, Moreno-Flores M, Avila J, Wandosell F, Minichiello L, Diaz-Nido J . BDNF production by olfactory ensheathing cells contributes to axonal regeneration of cultured adult CNS neurons. Neurochem Int. 2006; 50(3):491-8. DOI: 10.1016/j.neuint.2006.10.004. View

10.

Collins P, Tsang W, Urbanski H . Endocrine correlates of reproductive development in the male tree-shrew (Tupaia belangeri) and the effects of infantile exposure to exogenous androgens. Gen Comp Endocrinol. 2007; 154(1-3):22-30. DOI: 10.1016/j.ygcen.2007.06.024. View

11.

Andrews M, Stelzner D . Evaluation of olfactory ensheathing and schwann cells after implantation into a dorsal injury of adult rat spinal cord. J Neurotrauma. 2007; 24(11):1773-92. DOI: 10.1089/neu.2007.0353. View

12.

Diogo R . The head and neck muscles of the Philippine colugo (Dermoptera: Cynocephalus volans), with a comparison to tree-shrews, primates, and other mammals. J Morphol. 2008; 270(1):14-51. DOI: 10.1002/jmor.10666. View

13.

Lubelchek R, Max B, Sandusky C, Hota B, Barker D . Reliability at the lower limits of HIV-1 RNA quantification in clinical samples: a comparison of RT-PCR versus bDNA assays. PLoS One. 2009; 4(6):e6008. PMC: 2696104. DOI: 10.1371/journal.pone.0006008. View

14.

Zambello E, Fuchs E, Abumaria N, Rygula R, Domenici E, Caberlotto L . Chronic psychosocial stress alters NPY system: different effects in rat and tree shrew. Prog Neuropsychopharmacol Biol Psychiatry. 2009; 34(1):122-30. DOI: 10.1016/j.pnpbp.2009.10.011. View

15.

Gleiss A, Sanchez-Cabo F, Perco P, Tong D, Heinze G . Adaptive trimmed t-statistics for identifying predominantly high expression in a microarray experiment. Stat Med. 2010; 30(1):52-61. DOI: 10.1002/sim.4093. View

16.

Huo S, Li Y, Raisman G, Yin Z . Transplanted olfactory ensheathing cells reduce the gliotic injury response of Müller cells in a rat model of retinitis pigmentosa. Brain Res. 2011; 1382:238-44. DOI: 10.1016/j.brainres.2010.12.079. View

17.

Sasaki M, Lankford K, Radtke C, Honmou O, Kocsis J . Remyelination after olfactory ensheathing cell transplantation into diverse demyelinating environments. Exp Neurol. 2011; 229(1):88-98. DOI: 10.1016/j.expneurol.2011.01.010. View

18.

Xu Y, Kock J, Lu Y, Yang D, Lu M, Zhao X . Suppression of hepatitis B virus replication in Tupaia hepatocytes by tumor necrosis factor alpha of Tupaia belangeri. Comp Immunol Microbiol Infect Dis. 2011; 34(4):361-8. DOI: 10.1016/j.cimid.2011.05.003. View

19.

Aranda P, LaJoie D, Jorcyk C . Bleach gel: a simple agarose gel for analyzing RNA quality. Electrophoresis. 2012; 33(2):366-9. PMC: 3699176. DOI: 10.1002/elps.201100335. View

20.

Li S, Lee W, Zhang Y . Two bacterial infection models in tree shrew for evaluating the efficacy of antimicrobial agents. Dongwuxue Yanjiu. 2012; 33(1):1-6. DOI: 10.3724/SP.J.1141.2012.01001. View