A Novel Alphabaculovirus from the Soybean Looper, , That Produces Tetrahedral Occlusion Bodies and Encodes Two Copies of

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2019 Jun 29

PMID 31247912

Citations 2

Authors

Robert L Harrison

Daniel L Rowley

Holly J R Popham

Affiliations

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Abstract

Isolates of the alphabaculovirus species, , have been identified that produce polyhedral occlusion bodies and infect larvae of the soybean looper, . In this study, we report the discovery and characterization of a novel -infecting alphabaculovirus, Chrysodeixis includens nucleopolyhedrovirus #1 (ChinNPV#1), that produces tetrahedral occlusion bodies. In bioassays against larvae, ChinNPV #1 exhibited a degree of pathogenicity that was similar to that of other ChinNPV isolates, but killed larvae more slowly. The host range of ChinNPV#1 was found to be very narrow, with no indication of infection occurring in larvae of and six other noctuid species. The ChinNPV#1 genome sequence was determined to be 130,540 bp, with 126 open reading frames (ORFs) annotated but containing no homologous repeat () regions. Phylogenetic analysis placed ChinNPV#1 in a clade with other Group II alphabaculoviruses from hosts of lepidopteran subfamily Plusiinae, including Chrysodeixis chalcites nucleopolyhedrovirus and Trichoplusia ni single nucleopolyhedrovirus. A unique feature of the ChinNPV#1 genome was the presence of two full-length copies of the ORF. The results indicate that ChinNPV#1 is related to, but distinct from, other ChinNPV isolates.

Citing Articles

Ecological interactions, host plant defenses, and control strategies in managing soybean looper, (Lepidoptera: Noctuidae).

Debnath R, George J, Gautam M, Shafi I, Kariyat R, Reddy G Front Insect Sci. 2024; 4:1480940.

PMID: 39726917 PMC: 11669695. DOI: 10.3389/finsc.2024.1480940.

Complete Genome Sequences of Seven New Chrysodeixis includens Nucleopolyhedrovirus Isolates from Minas Gerais and Mato Grosso States in Brazil.

Craveiro S, Inglis P, Monteiro L, Santos L, Togawa R, Ribeiro Z Microbiol Resour Announc. 2020; 9(8).

PMID: 32079633 PMC: 7033270. DOI: 10.1128/MRA.01501-19.

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