Activation of Human Peripheral Blood Monocytes by Lipoproteins

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 1988 Feb 1

PMID 3124626

Citations 10

Authors

J L Kelley

M M Rozek

C A Suenram

C J Schwartz

Affiliations

Soon will be listed here.

Abstract

Activation of human peripheral blood monocytes could enhance their attachment and or migration into the arterial intima and their various secretory and other functions, thus influencing the pathogenesis of atherosclerosis. In these experiments the authors have explored the role of lipoproteins in the activation of human blood monocytes. Monocytes were purified from citrated blood by Histopaque density gradient centrifugation and countercurrent centrifugal elutriation and cultured in DMEM in the presence of 20% acid-treated autologous serum or 100 micrograms/ml each of VLDL, LDL, Ac-LDL, and HDL. Secretion of beta-glucuronidase activity into the media was measured as a marker of activation. All of the lipoprotein density classes as well as serum stimulated secretion of beta-glucuronidase activity, with LDL and Ac-LDL having a greater influence than serum, VLDL, or HDL. Serum and LDL also stimulated secretion of prostaglandin E into the culture medium. Incubation of monocytes with serum or LDL in the presence of inhibitors of arachidonate metabolism (NDGA and indomethacin) resulted in a significant decrease in secreted and intracellular beta-glucuronidase activity, indicating a role for products of arachidonate metabolism in the activation of monocytes by lipoproteins.

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References

WOOLLEN J, Walker P . The fluorimetric estimation of beta-glucuronidase in blood plasma. Clin Chim Acta. 1965; 12(6):659-70. DOI: 10.1016/0009-8981(65)90148-8. View

COHN Z, Benson B . THE DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. MORPHOLOGY, CYTOCHEMISTRY, AND BIOCHEMISTRY. J Exp Med. 1965; 121:153-70. PMC: 2137972. DOI: 10.1084/jem.121.1.153. View

Boyum A . Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968; 97:77-89. View

Mackaness G . The influence of immunologically committed lymphoid cells on macrophage activity in vivo. J Exp Med. 1969; 129(5):973-92. PMC: 2138649. DOI: 10.1084/jem.129.5.973. View

Werb Z, Gordon S . Secretion of a specific collagenase by stimulated macrophages. J Exp Med. 1975; 142(2):346-60. PMC: 2189891. DOI: 10.1084/jem.142.2.346. View

Kelley J, Alaupovic P . Lipid transport in the avian species. Part I. Isolation and characterization of apolipoproteins and major lipoprotein density classes of male turkey serum. Atherosclerosis. 1976; 24(1-2):155-75. DOI: 10.1016/0021-9150(76)90073-3. View

Sanderson R, Shepperdson R, VATTER A, TALMAGE D . Isolation and enumeration of peripheral blood monocytes. J Immunol. 1977; 118(4):1409-14. View

Schorlemmer H, Burger R, Hylton W, Allison A . Induction of lysosomal enzyme release from cultured macrophages by dextran sulfate. Clin Immunol Immunopathol. 1977; 7(1):88-96. DOI: 10.1016/0090-1229(77)90033-2. View

Schorlemmer H, Davies P, Hylton W, Gugig M, Allison A . The selective release of lysosomal acid hydrolases from mouse peritoneal macrophages by stimuli of chronic inflammation. Br J Exp Pathol. 1977; 58(3):315-26. PMC: 2041149. View

10.

van Ginkel C, Van Aken W, Oh J, Vreeken J . Stimulation of monocyte procoagulant activity by adherence to different surfaces. Br J Haematol. 1977; 37(1):35-45. View

11.

Kurland J, Bockman R . Prostaglandin E production by human blood monocytes and mouse peritoneal macrophages. J Exp Med. 1978; 147(3):952-7. PMC: 2184186. DOI: 10.1084/jem.147.3.952. View

12.

Schnyder J, Baggiolini M . Secretion of lysosomal hydrolases by stimulated and nonstimulated macrophages. J Exp Med. 1978; 148(2):435-50. PMC: 2184949. DOI: 10.1084/jem.148.2.435. View

13.

Bonney R, Wightman P, Davies P, Sadowski S, KUEHL Jr F, Humes J . Regulation of prostaglandin synthesis and of the selective release of lysosomal hydrolases by mouse peritoneal macrophages. Biochem J. 1978; 176(2):433-42. PMC: 1186251. DOI: 10.1042/bj1760433. View

14.

Passwell J, Dayer J, Merler E . Increased prostaglandin production by human monocytes after membrane receptor activation. J Immunol. 1979; 123(1):115-20. View

15.

Fogelman A, Seager J, Hokom M, Edwards P . Separation of and cholesterol synthesis by human lymphocytes and monocytes. J Lipid Res. 1979; 20(3):379-88. View

16.

Drapier J, Tenu J, Lemaire G, PETIT J . Regulation of plasminogen activator secretion in mouse peritoneal macrophages. I. - Role of serum studied by a new spectrophotometric assay for plasminogen activators. Biochimie. 1979; 61(4):463-71. DOI: 10.1016/s0300-9084(79)80202-3. View

17.

Brown M, Goldstein J, Krieger M, Ho Y, Anderson R . Reversible accumulation of cholesteryl esters in macrophages incubated with acetylated lipoproteins. J Cell Biol. 1979; 82(3):597-613. PMC: 2110476. DOI: 10.1083/jcb.82.3.597. View

18.

Ridge S, Oronsky A, Kerwar S . Induction of the synthesis of latent collagenase and latent neutral protease in chondrocytes by a factor synthesized by activated macrophages. Arthritis Rheum. 1980; 23(4):448-54. DOI: 10.1002/art.1780230407. View

19.

Brown M, Basu S, Falck J, Ho Y, Goldstein J . The scavenger cell pathway for lipoprotein degradation: specificity of the binding site that mediates the uptake of negatively-charged LDL by macrophages. J Supramol Struct. 1980; 13(1):67-81. DOI: 10.1002/jss.400130107. View

20.

Hoover R, Folger R, Haering W, Ware B, Karnovsky M . Adhesion of leukocytes to endothelium: roles of divalent cations, surface charge, chemotactic agents and substrate. J Cell Sci. 1980; 45:73-86. DOI: 10.1242/jcs.45.1.73. View