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Generating Tissue-engineered Intestinal Epithelium from Cultured Lgr5 Stem Cells

Overview
Journal Regen Ther
Date 2019 Jun 28
PMID 31245500
Citations 1
Authors
Zhassulan Baimakhanov
Yasuhiro Torashima
Akihiko Soyama
Yusuke Inoue
Yusuke Sakai
Mitsuhisa Takatsuki
Fumihiko Fujita
Kengo Kanetaka
Tamotsu Kuroki
Susumu Eguchi
Affiliations
Soon will be listed here.
Abstract

Introduction: Generating tissue-engineered small intestine (TESI) from mature intestinal cells has been established in a mouse model. The purpose of this study was to generate TESI from Lgr5 stem cells .

Methods: We used Lgr5-EGFP mice for intestinal crypt isolation. After seven days, cultured crypts with Lgr5 stem cells were seeded onto a biodegradable polymer and implanted into omentum of NOD/SCID mice.

Results: Engineered intestinal epithelium was generated from Lgr5 stem cells after four weeks of implantation. Intestinal epithelium was immunohistochemically positive for Paneth cells, enteroendocrine cells, goblet cells, microvilli of the absorptive enterocytes and Ki67.

Conclusion: Our observations suggest that transplanted Lgr5 stem cells can differentiate into the intestinal epithelium with further proliferative activity.

Citing Articles

Clinical challenges of short bowel syndrome and the path forward for organoid-based regenerative medicine.

Endo R, Sugimoto S, Shirosaki K, Kato H, Wada M, Kanai T Regen Ther. 2023; 24:64-73.

PMID: 37868721 PMC: 10584670. DOI: 10.1016/j.reth.2023.06.001.

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