Oral Administration of Expressing a 3D8 Single-chain Variable Fragment (scFv) Enhances Chicken Growth and Conserves Immune Homeostasis

Overview

Journal 3 Biotech

Publisher Springer

Specialty Biotechnology

Date 2019 Jun 28

PMID 31245246

Citations 6

Authors

Shanmugam Sureshkumar

Sun Keun Jung

Dongjun Kim

Keon Bong Oh

Hyeon Yang

Hwi Cheul Lee

Jo Yong Jin

Lee Hae Sun

Sukchan Lee

Sung June Byun

Affiliations

Soon will be listed here.

Abstract

The present study was aimed to investigate the effects of 3D8 scFv-secreting Probiotic () on growth performance, inflammatory responses, and intestinal microbial flora in chickens. To this end, a total of 14 healthy wild-type chickens were divided into two experimental groups. Each group was orally administrated with a daily dose of 10 colony-forming units (CFU) of 3D8 scFv-producing or wild-type (WT) for 35 days. Administration of /3D8 scFv significantly improved the body weight of chickens when compared to /WT group. The bacterial taxonomic composition of the fecal microbiota was determined by pyrosequencing of 16S rRNA gene amplicons. , and were dominant phyla in two experimental groups. However, in 3D8 treatment groups at genus level, the was highly abundant, being represented by 18.12%. In addition, serum levels of primary cytokines such as IL-6, IL-8, TNF-α, IFN-γ, IL-4, and IGF1 were markedly reduced in the probiotic 3D8 group. In summary, our results indicate that the administration of expressing 3D8 scFv has a modulatory effect on inflammatory responses, improves weight gain while not affecting the common microbial composition of the chicken intestine.

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