Separate DNA Sequences Are Required for Normal Female and Ecdysone-induced Male Expression of Drosophila Melanogaster Yolk Protein 1

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1987 Nov 1

PMID 3123886

Citations 8

Authors

A D Shirras

M Bownes

Affiliations

Soon will be listed here.

Abstract

Drosophila melanogaster flies were transformed with a yp1-Adh fusion gene with 890 bp of yp1 5' flanking sequence. In an Adh- background these flies show a stage, tissue and sex-specific pattern of alcohol dehydrogenase (ADH) activity characteristic of yolk protein genes. ADH activity is not present in dsxD/dsx pseudomales indicating that this fragment contains sites where the dsx gene product exerts its effect. Transformed male flies do not exhibit ADH activity when injected with 20-hydroxyecdysone while synthesis of native yolk proteins is induced. Thus the hormone inducibility and sex regulation have been separated in this construct.

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