Mild Hydrothermal Pretreatment of Sugarcane Bagasse Enhances the Production of Holocellulases by Aspergillus Niger

Overview

Journal J Ind Microbiol Biotechnol

Publisher Oxford University Press

Specialty Biotechnology

Date 2019 Jun 26

PMID 31236777

Citations 3

Authors

Caio de Oliveira Gorgulho Silva

Agenor de Castro Moreira Dos Santos Junior

Renata Henrique Santana

Ricardo Henrique Kruger

Wagner Fontes

Marcelo Valle de Sousa

Carlos Andre Ornelas Ricart

Edivaldo Ximenes Ferreira Filho

Affiliations

Soon will be listed here.

Abstract

Holocellulase production by Aspergillus niger using raw sugarcane bagasse (rSCB) as the enzyme-inducing substrate is hampered by the intrinsic recalcitrance of this material. Here we report that mild hydrothermal pretreatment of rSCB increases holocellulase secretion by A. niger. Quantitative proteomic analysis revealed that pretreated solids (PS) induced a pronounced up-regulation of endoglucanases and cellobiohydrolases compared to rSCB, which resulted in a 10.1-fold increase in glucose release during SCB saccharification. The combined use of PS and pretreatment liquor (PL), referred to as whole pretreated slurry (WPS), as carbon source induced a more balanced up-regulation of cellulases, hemicellulases and pectinases and resulted in the highest increase (4.8-fold) in the release of total reducing sugars from SCB. The use of PL as the sole carbon source induced the modulation of A. niger's secretome towards hemicellulose degradation. Mild pretreatment allowed the use of PL in downstream biological operations without the need for undesirable detoxification steps.

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