Effects of Puerarin on the Pharmacokinetics of Triptolide in Rats

Overview

Journal Pharm Biol

Specialties Pharmacology
Pharmacy

Date 2019 Jun 25

PMID 31230510

Citations 5

Authors

Qingfa Wang

Yanping Wu

Fengting Xiang

Yan Feng

Zhenghao Li

Yufeng Ding

Affiliations

Soon will be listed here.

Abstract

Puerarin and triptolide are sometimes used together for the treatment of disease in Chinese clinics; however, the drug-drug interaction between puerarin and triptolide is still unknown. This study investigates the effects of puerarin on the pharmacokinetics of triptolide in rats and clarifies its main mechanism. The pharmacokinetic profiles of oral administration of triptolide (1 mg/kg) in Sprague-Dawley rats with (test group, = 6) or without pretreatment (control group, = 6) with puerarin (100 mg/kg/day for seven days) were investigated. The effects of puerarin on the transport and metabolic stability of triptolide were also investigated using Caco-2 cell transwell model and rat liver microsomes. The results showed that puerarin could significantly increase the peak plasma concentration (from 187.25 ± 15.36 to 219.67 ± 21.52 ng/mL), and decrease its oral clearance (from 4.92 ± 0.35 to 62.46 ± 3.75 ± 0.19 L/h/kg). The Caco-2 cell transwell experiments indicated that puerarin could decrease the efflux ratio of triptolide from 2.70 to 1.33, and the intrinsic clearance rate of triptolide was decreased by the pretreatment with puerarin (38.8 ± 4.7 vs. 32.9 ± 6.5 μL/min/mg protein). Puerarin could significantly change the pharmacokinetic profiles of triptolide in rats, and it might exert these effects through increasing the absorption of triptolide by inhibiting the activity of , or through inhibiting the metabolism of triptolide in rat liver. The results also showed that the dose of triptolide should be decreased when these drugs were co-administered.

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References

Choo M, Park E, Yoon H, Kim D . Antithrombotic and antiallergic activities of daidzein, a metabolite of puerarin and daidzin produced by human intestinal microflora. Biol Pharm Bull. 2002; 25(10):1328-32. DOI: 10.1248/bpb.25.1328. View

Brinker A, Raskin I . Determination of triptolide in root extracts of Tripterygium wilfordii by solid-phase extraction and reverse-phase high-performance liquid chromatography. J Chromatogr A. 2005; 1070(1-2):65-70. DOI: 10.1016/j.chroma.2005.02.083. View

Yeung D, Leung S, Xu Y, Vanhoutte P, Man R . Puerarin, an isoflavonoid derived from Radix puerariae, potentiates endothelium-independent relaxation via the cyclic AMP pathway in porcine coronary artery. Eur J Pharmacol. 2006; 552(1-3):105-11. DOI: 10.1016/j.ejphar.2006.08.078. View

Jin M, Chen X, Ouyang X . Simultaneous determination of triptolide, tripdiolide and tripterine in human urine by high-performance liquid chromatography coupled with ion trap atmospheric-pressure chemical ionization mass spectrometry. Biomed Chromatogr. 2008; 23(3):289-94. DOI: 10.1002/bmc.1112. View

Wong K, Li G, Li K, Razmovski-Naumovski V, Chan K . Kudzu root: traditional uses and potential medicinal benefits in diabetes and cardiovascular diseases. J Ethnopharmacol. 2011; 134(3):584-607. DOI: 10.1016/j.jep.2011.02.001. View

Zhang H, Zhang L, Zhang Q, Yang X, Yu J, Shun S . Puerarin: a novel antagonist to inward rectifier potassium channel (IK1). Mol Cell Biochem. 2011; 352(1-2):117-23. DOI: 10.1007/s11010-011-0746-0. View

Liu Z, Ma L, Zhou G . The main anticancer bullets of the Chinese medicinal herb, thunder god vine. Molecules. 2011; 16(6):5283-97. PMC: 6264543. DOI: 10.3390/molecules16065283. View

Liu C, Ma J, Sun Y . Protective role of puerarin on lead-induced alterations of the hepatic glutathione antioxidant system and hyperlipidemia in rats. Food Chem Toxicol. 2011; 49(12):3119-27. DOI: 10.1016/j.fct.2011.09.007. View

Huang F, Liu K, Du H, Kou J, Liu B . Puerarin attenuates endothelial insulin resistance through inhibition of inflammatory response in an IKKβ/IRS-1-dependent manner. Biochimie. 2012; 94(5):1143-50. DOI: 10.1016/j.biochi.2012.01.018. View

10.

Hu Y, Tan Z, Wu X, Liu T, Jiang L, Bao R . Triptolide induces s phase arrest and apoptosis in gallbladder cancer cells. Molecules. 2014; 19(2):2612-28. PMC: 6271823. DOI: 10.3390/molecules19022612. View

11.

Wei D, Huang Z . Anti-inflammatory effects of triptolide in LPS-induced acute lung injury in mice. Inflammation. 2014; 37(4):1307-16. DOI: 10.1007/s10753-014-9858-5. View

12.

Kim S, Yoon I, Kim K, Cho S, Kim Y, Cho H . In vitro and in vivo evaluation of the effect of puerarin on hepatic cytochrome p450-mediated drug metabolism. Planta Med. 2014; 80(7):561-7. DOI: 10.1055/s-0034-1368350. View

13.

Liao Z, Liang X, Zhu J, Zhao G, Guan Y, Cao Y . Transport properties of puerarin and effect of extract of Radix Angelicae dahuricae on puerarin intestinal absorption using in situ and in vitro models. Phytother Res. 2014; 28(9):1288-94. DOI: 10.1002/ptr.5148. View

14.

Guo Y, Liang D, Xu Z, Ye Q . In vivo inhibitory effects of puerarin on selected rat cytochrome P450 isoenzymes. Pharmazie. 2014; 69(5):367-70. View

15.

Li X, Jiang Z, Zhang L . Triptolide: progress on research in pharmacodynamics and toxicology. J Ethnopharmacol. 2014; 155(1):67-79. DOI: 10.1016/j.jep.2014.06.006. View

16.

Ling D, Xia H, Park W, Hackett M, Song C, Na K . pH-sensitive nanoformulated triptolide as a targeted therapeutic strategy for hepatocellular carcinoma. ACS Nano. 2014; 8(8):8027-39. DOI: 10.1021/nn502074x. View

17.

Su P, Cheng Q, Wang X, Cheng X, Zhang M, Tong Y . Characterization of eight terpenoids from tissue cultures of the Chinese herbal plant, Tripterygium wilfordii, by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry. Biomed Chromatogr. 2014; 28(9):1183-92. DOI: 10.1002/bmc.3140. View

18.

Cheng S, LeBlanc K, Li L . Triptolide preserves cognitive function and reduces neuropathology in a mouse model of Alzheimer's disease. PLoS One. 2014; 9(9):e108845. PMC: 4183525. DOI: 10.1371/journal.pone.0108845. View

19.

Wang B, Cao J, Chen J, Liu Q . Triptolide induces apoptosis of gastric cancer cells via inhibiting the overexpression of MDM2. Med Oncol. 2014; 31(11):270. DOI: 10.1007/s12032-014-0270-7. View

20.

Singla N, Challana S . Reproductive toxicity of triptolide in male house rat, Rattus rattus. ScientificWorldJournal. 2014; 2014:879405. PMC: 4211135. DOI: 10.1155/2014/879405. View