Lipid-peptide Bioconjugation Through Pyridyl Disulfide Reaction Chemistry and Its Application in Cell Targeting and Drug Delivery

Overview

Journal J Nanobiotechnology

Publisher Biomed Central

Specialty Biotechnology

Date 2019 Jun 23

PMID 31226993

Citations 7

Authors

Diego de la Fuente-Herreruela

Ajay K Monnappa

Monica Munoz-Ubeda

Aaron Morallon-Pina

Eduardo Enciso

Luis Sanchez

Fabrice Giusti

Paolo Natale

Ivan Lopez-Montero

Affiliations

Soon will be listed here.

Abstract

Background: The design of efficient drug delivery vectors requires versatile formulations able to simultaneously direct a multitude of molecular targets and to bypass the endosomal recycling pathway of cells. Liposomal-based vectors need the decoration of the lipid surface with specific peptides to fulfill the functional requirements. The unspecific binding of peptides to the lipid surface is often accompanied with uncontrolled formulations and thus preventing the molecular mechanisms of a successful therapy.

Results: We present a simple synthesis pathway to anchor cysteine-terminal peptides to thiol-reactive lipids for adequate and quantitative liposomal formulations. As a proof of concept, we have synthesized two different lipopeptides based on (a) the truncated Fibroblast Growth Factor (tbFGF) for cell targeting and (b) the pH sensitive and fusogenic GALA peptide for endosomal scape.

Conclusions: The incorporation of these two lipopeptides in the liposomal formulation improves the fibroblast cell targeting and promotes the direct delivery of cargo molecules to the cytoplasm of the cell.

Citing Articles

Targeting Peptides: The New Generation of Targeted Drug Delivery Systems.

Todaro B, Ottalagana E, Luin S, Santi M Pharmaceutics. 2023; 15(6).

PMID: 37376097 PMC: 10300729. DOI: 10.3390/pharmaceutics15061648.

Lipid Nanoparticles and Liposomes for Bone Diseases Treatment.

Burdusel A, Andronescu E Biomedicines. 2022; 10(12).

PMID: 36551914 PMC: 9775639. DOI: 10.3390/biomedicines10123158.

Transgene expression in mice of the Opa1 mitochondrial transmembrane protein through bicontinuous cubic lipoplexes containing gemini imidazolium surfactants.

Munoz-Ubeda M, Semenzato M, Franco-Romero A, Junquera E, Aicart E, Scorrano L J Nanobiotechnology. 2021; 19(1):425.

PMID: 34922554 PMC: 8684174. DOI: 10.1186/s12951-021-01167-x.

Evaluating Endosomal Escape of Caspase-3-Containing Nanomaterials Using Split GFP.

Anson F, Liu B, Kanjilal P, Wu P, Hardy J, Thayumanavan S Biomacromolecules. 2021; 22(3):1261-1272.

PMID: 33591168 PMC: 8477791. DOI: 10.1021/acs.biomac.0c01767.

Recent Progress in Bioconjugation Strategies for Liposome-Mediated Drug Delivery.

Almeida B, Nag O, Rogers K, Delehanty J Molecules. 2020; 25(23).

PMID: 33271886 PMC: 7730700. DOI: 10.3390/molecules25235672.

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