The Relationship Between Insecticide Resistance, Mosquito Age and Malaria Prevalence in Anopheles Gambiae S.l. from Guinea

Overview

Journal Sci Rep

Specialty Science

Date 2019 Jun 22

PMID 31222175

Citations 29

Authors

Emma Collins

Natasha M Vaselli

Moussa Sylla

Abdoul H Beavogui

James Orsborne

Gena Lawrence

Ryan E Wiegand

Seth R Irish

Thomas Walker

Louisa A Messenger

Affiliations

Soon will be listed here.

Abstract

Insecticide resistance across sub-Saharan Africa may impact the continued effectiveness of malaria vector control. We investigated the association between carbamate and pyrethroid resistance with Anopheles gambiae s.l. parity, Plasmodium falciparum infection, and molecular insecticide resistance mechanisms in Guinea. Pyrethroid resistance was intense, with field populations surviving ten times the insecticidal concentration required to kill susceptible individuals. The L1014F kdr-N1575Y haplotype and I1527T mutation were significantly associated with mosquito survival following permethrin exposure (Prevalence Ratio; PR = 1.92, CI = 1.09-3.37 and PR = 2.80, CI = 1.03-7.64, respectively). Partial restoration of pyrethroid susceptibility following synergist pre-exposure suggests a role for mixed-function oxidases. Carbamate resistance was lower and significantly associated with the G119S Ace-1 mutation. Oocyst rates were 6.8% and 4.2% among resistant and susceptible mosquitoes, respectively; survivors of bendiocarb exposure were significantly more likely to be infected. Pyrethroid resistant mosquitoes had significantly lower parity rates than their susceptible counterparts (PR = 1.15, CI = 1.10-1.21). Our findings emphasize the need for additional studies directly assessing the influence of insecticide resistance on mosquito fitness.

Citing Articles

Omics Approaches in Understanding Insecticide Resistance in Mosquito Vectors.

Bharadwaj N, Sharma R, Subramanian M, Ragini G, Nagarajan S, Rahi M Int J Mol Sci. 2025; 26(5).

PMID: 40076478 PMC: 11899280. DOI: 10.3390/ijms26051854.

Unpacking WHO and CDC Bottle Bioassay Methods: A Comprehensive Literature Review and Protocol Analysis Revealing Key Outcome Predictors.

Praulins G, Murphy-Fegan A, Gillespie J, Mechan F, Gleave K, Lees R Gates Open Res. 2024; 8:56.

PMID: 39170853 PMC: 11335745. DOI: 10.12688/gatesopenres.15433.1.

Genetic markers associated with the widespread insecticide resistance in malaria vector Anopheles funestus populations across Tanzania.

Odero J, Nambunga I, Masalu J, Mkandawile G, Bwanary H, Hape E Parasit Vectors. 2024; 17(1):230.

PMID: 38760849 PMC: 11100202. DOI: 10.1186/s13071-024-06315-4.

The impact of pyrethroid-pyriproxyfen and pyrethroid-chlorfenapyr long-lasting insecticidal nets on density of primary malaria vectors Anopheles gambiae s.s. and Anopheles coluzzii in Benin: a secondary analysis of a cluster randomised controlled....

Yovogan B, Sovi A, Djenontin A, Adoha C, Akinro B, Accrombessi M Parasit Vectors. 2024; 17(1):7.

PMID: 38178161 PMC: 10768265. DOI: 10.1186/s13071-023-06104-5.

Knockdown resistance allele L1014F introduced by CRISPR/Cas9 is not associated with altered vector competence of Anopheles gambiae for o'nyong nyong virus.

Kay G, Patterson E, Hughes G, Lord J, Reimer L PLoS One. 2023; 18(8):e0288994.

PMID: 37561739 PMC: 10414658. DOI: 10.1371/journal.pone.0288994.

References

Reimer L, Fondjo E, Patchoke S, Diallo B, Lee Y, Ng A . Relationship between kdr mutation and resistance to pyrethroid and DDT insecticides in natural populations of Anopheles gambiae. J Med Entomol. 2008; 45(2):260-6. DOI: 10.1603/0022-2585(2008)45[260:rbkmar]2.0.co;2. View

Henry M, Assi S, Rogier C, Dossou-Yovo J, Chandre F, Guillet P . Protective efficacy of lambda-cyhalothrin treated nets in Anopheles gambiae pyrethroid resistance areas of Côte d'Ivoire. Am J Trop Med Hyg. 2005; 73(5):859-64. View

Asidi A, NGuessan R, Akogbeto M, Curtis C, Rowland M . Loss of household protection from use of insecticide-treated nets against pyrethroid-resistant mosquitoes, benin. Emerg Infect Dis. 2012; 18(7):1101-6. PMC: 3376816. DOI: 10.3201/eid1807.120218. View

Hemingway J, Shretta R, Wells T, Bell D, Djimde A, Achee N . Tools and Strategies for Malaria Control and Elimination: What Do We Need to Achieve a Grand Convergence in Malaria?. PLoS Biol. 2016; 14(3):e1002380. PMC: 4774904. DOI: 10.1371/journal.pbio.1002380. View

Wilkins E, Howell P, Benedict M . IMP PCR primers detect single nucleotide polymorphisms for Anopheles gambiae species identification, Mopti and Savanna rDNA types, and resistance to dieldrin in Anopheles arabiensis. Malar J. 2006; 5:125. PMC: 1769388. DOI: 10.1186/1475-2875-5-125. View

Djogbenou L, Noel V, Agnew P . Costs of insensitive acetylcholinesterase insecticide resistance for the malaria vector Anopheles gambiae homozygous for the G119S mutation. Malar J. 2010; 9:12. PMC: 2816975. DOI: 10.1186/1475-2875-9-12. View

Lehmann T, Diabate A . The molecular forms of Anopheles gambiae: a phenotypic perspective. Infect Genet Evol. 2008; 8(5):737-46. PMC: 2731232. DOI: 10.1016/j.meegid.2008.06.003. View

Strode C, Donegan S, Garner P, Enayati A, Hemingway J . The impact of pyrethroid resistance on the efficacy of insecticide-treated bed nets against African anopheline mosquitoes: systematic review and meta-analysis. PLoS Med. 2014; 11(3):e1001619. PMC: 3958359. DOI: 10.1371/journal.pmed.1001619. View

Foley D, Harrison G, Murphy J, Dowler M, Rueda L, Wilkerson R . Mosquito bisection as a variable in estimates of PCR-derived malaria sporozoite rates. Malar J. 2012; 11:145. PMC: 3411414. DOI: 10.1186/1475-2875-11-145. View

10.

Riehle M, Bukhari T, Gneme A, Guelbeogo W, Coulibaly B, Fofana A . The 2La chromosome inversion is associated with susceptibility to in Africa. Elife. 2017; 6. PMC: 5482571. DOI: 10.7554/eLife.25813. View

11.

Toe K, NFale S, Dabire R, Ranson H, Jones C . The recent escalation in strength of pyrethroid resistance in Anopheles coluzzi in West Africa is linked to increased expression of multiple gene families. BMC Genomics. 2015; 16:146. PMC: 4352231. DOI: 10.1186/s12864-015-1342-6. View

12.

Marie A, Boissiere A, Tchioffo Tsapi M, Poinsignon A, Awono-Ambene P, Morlais I . Evaluation of a real-time quantitative PCR to measure the wild Plasmodium falciparum infectivity rate in salivary glands of Anopheles gambiae. Malar J. 2013; 12:224. PMC: 3707787. DOI: 10.1186/1475-2875-12-224. View

13.

Spillings B, Coetzee M, Koekemoer L, Brooke B . The effect of a single blood meal on the phenotypic expression of insecticide resistance in the major malaria vector Anopheles funestus. Malar J. 2008; 7:226. PMC: 2584071. DOI: 10.1186/1475-2875-7-226. View

14.

Alout H, Djegbe I, Chandre F, Djogbenou L, Dabire R, Corbel V . Insecticide exposure impacts vector-parasite interactions in insecticide-resistant malaria vectors. Proc Biol Sci. 2014; 281(1786). PMC: 4046407. DOI: 10.1098/rspb.2014.0389. View

15.

Hunt R, Brooke B, Pillay C, Koekemoer L, Coetzee M . Laboratory selection for and characteristics of pyrethroid resistance in the malaria vector Anopheles funestus. Med Vet Entomol. 2005; 19(3):271-5. DOI: 10.1111/j.1365-2915.2005.00574.x. View

16.

Ochomo E, Chahilu M, Cook J, Kinyari T, Bayoh N, West P . Insecticide-Treated Nets and Protection against Insecticide-Resistant Malaria Vectors in Western Kenya. Emerg Infect Dis. 2017; 23(5):758-764. PMC: 5403037. DOI: 10.3201/eid2305.161315. View

17.

Lines J, Nassor N . DDT resistance in Anopheles gambiae declines with mosquito age. Med Vet Entomol. 1991; 5(3):261-5. DOI: 10.1111/j.1365-2915.1991.tb00550.x. View

18.

Crawford J, Riehle M, Markianos K, Bischoff E, Guelbeogo W, Gneme A . Evolution of GOUNDRY, a cryptic subgroup of Anopheles gambiae s.l., and its impact on susceptibility to Plasmodium infection. Mol Ecol. 2016; 25(7):1494-510. DOI: 10.1111/mec.13572. View

19.

Harris A, Rajatileka S, Ranson H . Pyrethroid resistance in Aedes aegypti from Grand Cayman. Am J Trop Med Hyg. 2010; 83(2):277-84. PMC: 2911171. DOI: 10.4269/ajtmh.2010.09-0623. View

20.

Diop M, Moiroux N, Chandre F, Martin-Herrou H, Milesi P, Boussari O . Behavioral cost & overdominance in Anopheles gambiae. PLoS One. 2015; 10(4):e0121755. PMC: 4382092. DOI: 10.1371/journal.pone.0121755. View