Induction of Murine Cytolytic T Lymphocytes by Pseudomonas Aeruginosa Exotoxin A

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1988 Jan 1

PMID 3121510

Citations 5

Affiliations

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Abstract

Pseudomonas aeruginosa exotoxin A (PA), a potent protein synthesis inhibitor, was found to be a weak T-cell mitogen for murine splenocytes. Maximal stimulation of [3H]thymidine incorporation was obtained with 10 to 100 ng of toxin per ml following a 4-day induction. PA was also shown to be a polyclonal activator of cytolytic T lymphocytes (CTL), effective against concanavalin A-treated target cells. The effective PA dose for CTL induction was the same as that for mitogenic stimulation, only with a prolonged priming time (7 days). In contrast to other mitogens, PA could not reactivate memory CTL into secondary CTL. The stimulation of CTL by subcytotoxic doses of PA may be relevant to its modulatory effect on the immunocellular system.

Citing Articles

In vivo production of exotoxin A and its role in endogenous Pseudomonas aeruginosa septicemia in mice.

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Detection of Pseudomonas aeruginosa from clinical and environmental samples by amplification of the exotoxin A gene using PCR.

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Induction of inflammatory mediators (histamine and leukotrienes) from rat peritoneal mast cells and human granulocytes by Pseudomonas aeruginosa strains from burn patients.

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In vivo protective effect of lipopolysaccharide against Pseudomonas aeruginosa exotoxin A in mice.

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