Association Between Sheath Blight Resistance and Chitinase Activity in Transgenic Rice Plants Expressing McCHIT1 from Bitter Melon

Overview

Journal Transgenic Res

Specialty Molecular Biology

Date 2019 Jun 20

PMID 31214892

Citations 5

Authors

Changwei Zhang

Manyu Huang

Xianchun Sang

Ping Li

Yinghua Ling

Fangming Zhao

Dan Du

Yunfeng Li

Zhenglin Yang

Guanghua He

Affiliations

Soon will be listed here.

Abstract

No usable resources with high-level resistance to sheath blight (SB) have yet been found in rice germplasm resources worldwide. Therefore, creating and breeding new disease-resistant rice resources with sheath blight resistance (SBR) are imperative. In this study, we inoculated rice plants with hyphae of the highly pathogenic strain RH-9 of rice SB fungus Rhizoctonia solani to obtain eight stable transgenic rice lines harbouring the chitinase gene (McCHIT1) of bitter melon with good SBR in the T generation. The mean disease index for SB of wild-type plants was 92% and 37-44% in transgenic lines. From 24 h before until 120 h after inoculation with R. solani, chitinase activity in stable transgenic plants with increased SBR was 2.0-5.5 and 1.8-2.7 times that of wild-type plants and plants of a disease-susceptible stable transgenic line, respectively. The correlation between SBR and chitinase activity in McCHIT1-transgenic rice line plants was significant. This work stresses how McCHIT1 from bitter melon can be used to protect rice plants from SB infection.

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