High-Fat Diet Alters the Intestinal Microbiota in Streptozotocin-Induced Type 2 Diabetic Mice

Overview

Journal Microorganisms

Specialty Microbiology

Date 2019 Jun 19

PMID 31208113

Citations 30

Authors

Sheng Liu

Panpan Qin

Jing Wang

Affiliations

Soon will be listed here.

Abstract

Intestinal microbiota is closely associated with various metabolic diseases such as type 2 diabetes (T2D), and microbiota is definitely affected by diet. However, more work is required to gain detailed information about gut metagenome and their associated impact with diet in T2D patients. We used a streptozotocin-high-fat diet (HFD) to induce a T2D mouse model and investigated the effect of standard chow diet and HFD on the composition and function of gut microbiota. We found that a HFD could worsen the diabetes status compared with a standard diet. 16S rRNA gene sequencing revealed that a HFD caused a large disturbance to the microbial structure and was linked to an increased ratio of to . A HFD increased the bacteria of the and family and decreased the bacteria of and . Meanwhile, a HFD decreased the abundance of and , both of which have previously been reported to alleviate obesity and metabolic dysfunctions. Moreover, PICRUSt-predicted KEGG pathways related to membrane transport, lipid metabolism, and xenobiotics biodegradation and metabolism were significantly elevated in HFD-fed T2D mice. Our results provide insights into dietary and nutritional approaches for improving host metabolism and ameliorating T2D.

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References

Kageyama A, Benno Y . Coprobacillus catenaformis gen. nov., sp. nov., a new genus and species isolated from human feces. Microbiol Immunol. 2000; 44(1):23-8. DOI: 10.1111/j.1348-0421.2000.tb01242.x. View

Patil K, Nielsen J . Uncovering transcriptional regulation of metabolism by using metabolic network topology. Proc Natl Acad Sci U S A. 2005; 102(8):2685-9. PMC: 549453. DOI: 10.1073/pnas.0406811102. View

Eckburg P, Bik E, Bernstein C, Purdom E, Dethlefsen L, Sargent M . Diversity of the human intestinal microbial flora. Science. 2005; 308(5728):1635-8. PMC: 1395357. DOI: 10.1126/science.1110591. View

Srinivasan K, Viswanad B, Asrat L, Kaul C, Ramarao P . Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: a model for type 2 diabetes and pharmacological screening. Pharmacol Res. 2005; 52(4):313-20. DOI: 10.1016/j.phrs.2005.05.004. View

Cefalu W . Animal models of type 2 diabetes: clinical presentation and pathophysiological relevance to the human condition. ILAR J. 2006; 47(3):186-98. DOI: 10.1093/ilar.47.3.186. View

DeSantis T, Hugenholtz P, Larsen N, Rojas M, Brodie E, Keller K . Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Appl Environ Microbiol. 2006; 72(7):5069-72. PMC: 1489311. DOI: 10.1128/AEM.03006-05. View

Lozupone C, Hamady M, Knight R . UniFrac--an online tool for comparing microbial community diversity in a phylogenetic context. BMC Bioinformatics. 2006; 7:371. PMC: 1564154. DOI: 10.1186/1471-2105-7-371. View

Ley R, Turnbaugh P, Klein S, Gordon J . Microbial ecology: human gut microbes associated with obesity. Nature. 2006; 444(7122):1022-3. DOI: 10.1038/4441022a. View

Turnbaugh P, Ley R, Mahowald M, Magrini V, Mardis E, Gordon J . An obesity-associated gut microbiome with increased capacity for energy harvest. Nature. 2006; 444(7122):1027-31. DOI: 10.1038/nature05414. View

10.

Backhed F, Manchester J, Semenkovich C, Gordon J . Mechanisms underlying the resistance to diet-induced obesity in germ-free mice. Proc Natl Acad Sci U S A. 2007; 104(3):979-84. PMC: 1764762. DOI: 10.1073/pnas.0605374104. View

11.

Turnbaugh P, Backhed F, Fulton L, Gordon J . Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome. Cell Host Microbe. 2008; 3(4):213-23. PMC: 3687783. DOI: 10.1016/j.chom.2008.02.015. View

12.

Hildebrandt M, Hoffmann C, Sherrill-Mix S, Keilbaugh S, Hamady M, Chen Y . High-fat diet determines the composition of the murine gut microbiome independently of obesity. Gastroenterology. 2009; 137(5):1716-24.e1-2. PMC: 2770164. DOI: 10.1053/j.gastro.2009.08.042. View

13.

Schloss P, Westcott S, Ryabin T, Hall J, Hartmann M, Hollister E . Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol. 2009; 75(23):7537-41. PMC: 2786419. DOI: 10.1128/AEM.01541-09. View

14.

Qin J, Li R, Raes J, Arumugam M, Burgdorf K, Manichanh C . A human gut microbial gene catalogue established by metagenomic sequencing. Nature. 2010; 464(7285):59-65. PMC: 3779803. DOI: 10.1038/nature08821. View

15.

Sato A, Kawano H, Notsu T, Ohta M, Nakakuki M, Mizuguchi K . Antiobesity effect of eicosapentaenoic acid in high-fat/high-sucrose diet-induced obesity: importance of hepatic lipogenesis. Diabetes. 2010; 59(10):2495-504. PMC: 3279525. DOI: 10.2337/db09-1554. View

16.

Goodman A, Kallstrom G, Faith J, Reyes A, Moore A, Dantas G . Extensive personal human gut microbiota culture collections characterized and manipulated in gnotobiotic mice. Proc Natl Acad Sci U S A. 2011; 108(15):6252-7. PMC: 3076821. DOI: 10.1073/pnas.1102938108. View

17.

Serino M, Luche E, Gres S, Baylac A, Berge M, Cenac C . Metabolic adaptation to a high-fat diet is associated with a change in the gut microbiota. Gut. 2011; 61(4):543-53. PMC: 3292714. DOI: 10.1136/gutjnl-2011-301012. View

18.

Berry D, Schwab C, Milinovich G, Reichert J, Ben Mahfoudh K, Decker T . Phylotype-level 16S rRNA analysis reveals new bacterial indicators of health state in acute murine colitis. ISME J. 2012; 6(11):2091-106. PMC: 3475367. DOI: 10.1038/ismej.2012.39. View

19.

Million M, Angelakis E, Paul M, Armougom F, Leibovici L, Raoult D . Comparative meta-analysis of the effect of Lactobacillus species on weight gain in humans and animals. Microb Pathog. 2012; 53(2):100-8. DOI: 10.1016/j.micpath.2012.05.007. View

20.

Junemann S, Prior K, Szczepanowski R, Harks I, Ehmke B, Goesmann A . Bacterial community shift in treated periodontitis patients revealed by ion torrent 16S rRNA gene amplicon sequencing. PLoS One. 2012; 7(8):e41606. PMC: 3411582. DOI: 10.1371/journal.pone.0041606. View