Targeted Transcript Analysis Revealed Association of Suboptimal Expression of Certain Endometrial Immunity-related Genes with Disparate Uterine Diseases in Zebu Cows

Overview

Journal Trop Anim Health Prod

Publisher Springer

Specialties Tropical Medicine
Veterinary Medicine

Date 2019 Jun 15

PMID 31197726

Citations 2

Authors

R K Baithalu

S K Singh

A Kumaresan

S Kumar

B R Maharana

S Mallick

T K Mohanty

A K Mohanty

Affiliations

Soon will be listed here.

Abstract

The onset of uterine infection during postpartum period compromises uterine health, fertility, and productivity of dairy cattle. Endometrial innate immunity plays a key role in eliminating uterine infection and keeping the uterus healthy. Hence, the present study has been designed with the hypothesis that altered endometrial immune response around calving may compromise uterine health during postpartum period. Expression of interleukins (IL-1β, IL-6, IL-8, IL-10, and TNF-α), prostaglandin synthase (PGFS, PGES), and antimicrobial peptides (beta-defensins (BDEF-4, BDEF-5), lingual antimicrobial peptide (LAP), and calcium-binding proteins (S100A8, S100A9, and S100A12) in endometrial tissues on the day of calving was studied using qRT-PCR, and circulating concentrations of prostaglandin E and F metabolites (PGEM and PGFM) during peripartum period (on days - 7, - 4, - 1 (before calving), 0 (on the day of calving), + 1, + 4, and + 7 (post calving)) of normal (healthy) cows (n = 11) that did not develop postpartum uterine infection and cows that developed puerperal metritis (n = 7) and clinical endometritis (n = 6) were studied. Endometrial expression of IL-1β, TNF-α, BDEF-4, BDEF-5, S100A8, S100A12, and PGFS was higher (P < 0.05), and expression of IL-6, IL-8, IL-10, and PGES was lower (P < 0.05) in normal (healthy) cows than puerperal metritic and clinical endometritic cows. The PGFM concentration in serum was high (P < 0.05) on days 0, + 1, and + 4 of calving in puerperal metritic cows followed by normal and clinical endometritic cows. However, PGEM concentration in serum was high (P < 0.05) during peripartum period in uterine-infected (puerperal metritic and clinical endometritic) cows compared with normal cows. From the above findings, it is concluded that higher constitutive expression of IL-1β, TNF-α, PGFS, BDEF-4, BDEF-5, S100A8, and S100A12 genes in the endometrium and lower concentration of PGEM during the period immediate to calving might be beneficial for uterine health of cows.

Citing Articles

GR-1 Alleviates -Induced Inflammation NF-κB and MAPKs Signaling in Bovine Endometrial Epithelial Cells.

Liu J, Feng X, Li B, Sun Y, Jin T, Feng M Front Cell Infect Microbiol. 2022; 12:809674.

PMID: 35310848 PMC: 8924357. DOI: 10.3389/fcimb.2022.809674.

S100A8 expression in oviduct mucosal epithelial cells is regulated by estrogen and affects mucosal immune homeostasis.

Li X, Cao G, Yang H, Zhi D, Li L, Wang D PLoS One. 2021; 16(11):e0260188.

PMID: 34793556 PMC: 8601440. DOI: 10.1371/journal.pone.0260188.

References

Akira S, Takeda K . Toll-like receptor signalling. Nat Rev Immunol. 2004; 4(7):499-511. DOI: 10.1038/nri1391. View

Baithalu R, Singh S, Kumaresan A, Mohanty A, Mohanty T, Kumar S . Transcriptional abundance of antioxidant enzymes in endometrium and their circulating levels in Zebu cows with and without uterine infection. Anim Reprod Sci. 2016; 177:79-87. DOI: 10.1016/j.anireprosci.2016.12.008. View

Beutler B . Inferences, questions and possibilities in Toll-like receptor signalling. Nature. 2004; 430(6996):257-63. DOI: 10.1038/nature02761. View

Chapwanya A, Meade K, Doherty M, Callanan J, Mee J, OFarrelly C . Histopathological and molecular evaluation of Holstein-Friesian cows postpartum: toward an improved understanding of uterine innate immunity. Theriogenology. 2009; 71(9):1396-407. DOI: 10.1016/j.theriogenology.2009.01.006. View

Cormican P, Meade K, Cahalane S, Narciandi F, Chapwanya A, Lloyd A . Evolution, expression and effectiveness in a cluster of novel bovine beta-defensins. Immunogenetics. 2008; 60(3-4):147-56. DOI: 10.1007/s00251-007-0269-8. View

Davies D, Meade K, Herath S, Eckersall P, Gonzalez D, White J . Toll-like receptor and antimicrobial peptide expression in the bovine endometrium. Reprod Biol Endocrinol. 2008; 6:53. PMC: 2627908. DOI: 10.1186/1477-7827-6-53. View

Gabler C, Fischer C, Drillich M, Einspanier R, Heuwieser W . Time-dependent mRNA expression of selected pro-inflammatory factors in the endometrium of primiparous cows postpartum. Reprod Biol Endocrinol. 2010; 8:152. PMC: 3016299. DOI: 10.1186/1477-7827-8-152. View

Galvao K, Santos N, Galvao J, Gilbert R . Association between endometritis and endometrial cytokine expression in postpartum Holstein cows. Theriogenology. 2011; 76(2):290-9. DOI: 10.1016/j.theriogenology.2011.02.006. View

Havelock J, Keller P, Muleba N, Mayhew B, Casey B, Rainey W . Human myometrial gene expression before and during parturition. Biol Reprod. 2004; 72(3):707-19. DOI: 10.1095/biolreprod.104.032979. View

10.

Herath S, Fischer D, Werling D, Williams E, T Lilly S, Dobson H . Expression and function of Toll-like receptor 4 in the endometrial cells of the uterus. Endocrinology. 2005; 147(1):562-70. PMC: 2738982. DOI: 10.1210/en.2005-1113. View

11.

Herath S, T Lilly S, Santos N, Gilbert R, Goetze L, Bryant C . Expression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertility. Reprod Biol Endocrinol. 2009; 7:55. PMC: 2702306. DOI: 10.1186/1477-7827-7-55. View

12.

Hoedemaker M, Lund L, Wagner W . Influence of arachidonic acid metabolites and steroids on function of bovine polymorphonuclear neutrophils. Am J Vet Res. 1992; 53(9):1534-9. View

13.

Konigsson K, Gustafsson H, Kindahl H . 15-Ketodihydro-PGF(2 alpha), progesterone and uterine involution in primiparous cows with induced retained placenta and post-partal endometritis treated with oxytetracycline and flunixin. Reprod Domest Anim. 2002; 37(1):43-51. DOI: 10.1046/j.1439-0531.2002.00325.x. View

14.

Lehrer R . Primate defensins. Nat Rev Microbiol. 2004; 2(9):727-38. DOI: 10.1038/nrmicro976. View

15.

Leslie K . The events of normal and abnormal postpartum reproductive endocrinology and uterine involution in dairy cows: a review. Can Vet J. 1983; 24(3):67-71. PMC: 1790317. View

16.

Li Q, Verma I . NF-kappaB regulation in the immune system. Nat Rev Immunol. 2002; 2(10):725-34. DOI: 10.1038/nri910. View

17.

Lindell J, Kindahl H, Jansson L, Edqvist L . Post-partum release of prostaglandin F(2alpha) and uterine involution in the cow. Theriogenology. 1982; 17(3):237-45. DOI: 10.1016/0093-691x(82)90085-1. View

18.

Loyi T, Kumar H, Nandi S, Mathapati B, Patra M, Pattnaik B . Differential expression of pro-inflammatory cytokines in endometrial tissue of buffaloes with clinical and sub-clinical endometritis. Res Vet Sci. 2012; 94(2):336-40. DOI: 10.1016/j.rvsc.2012.09.008. View

19.

Christoffersen M, Mette C, Baagoe C, Camilla Dooleweerdt B, Jacobsen S, Stine J . Evaluation of the systemic acute phase response and endometrial gene expression of serum amyloid A and pro- and anti-inflammatory cytokines in mares with experimentally induced endometritis. Vet Immunol Immunopathol. 2010; 138(1-2):95-105. DOI: 10.1016/j.vetimm.2010.07.011. View

20.

Mijatovic T, Kruys V, Caput D, Defrance P, Huez G . Interleukin-4 and -13 inhibit tumor necrosis factor-alpha mRNA translational activation in lipopolysaccharide-induced mouse macrophages. J Biol Chem. 1997; 272(22):14394-8. DOI: 10.1074/jbc.272.22.14394. View