Galactosyltransferase and Sialyltransferase Are Located in Different Subcellular Compartments in HeLa Cells

Overview

Journal Exp Cell Res

Specialty Cell Biology

Date 1987 Nov 1

PMID 3119357

Citations 18

Authors

E G Berger

M Thurnher

U Muller

Affiliations

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Abstract

Galactosyl- and sialyltransferase have been localized by double immunofluorescence labeling in HeLa cells. Galactosyltransferase was found in a compact juxtanuclear structure previously shown to represent the Golgi apparatus (J. Roth and E.G. Berger (1982) J. Cell Biol. 93, 223-9), whereas sialyltransferase was localized to vesicles spread over the whole cytoplasm. These findings indicate different compartments for both transferases and support a model of subcompartmentation of glycosylation steps along the secretory pathway.

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