Article: Evaluation of genetic relationship among varieties of L. and L. in West Africa using ISSR markers

The taxonomic identity of two closely related species; and in West Africa has not been clarified because they have overlapping morphological traits. Effective control and management measures as well as improvement of crop plants in any breeding programme can only be implemented when plant species are correctly identified. The genetic relationships of the varieties of these species were assessed using 10 ISSR primers for the first time. The varieties and species used include var. ; var. ; var. and var. PCR amplification of the isolated DNA from the four varieties of revealed a total of 75 loci out of which 14 were found to be polymorphic. Average polymorphism information content (PIC) and heterozygosity (He) of the 10 ISSR markers were estimated as 0.67 and 0.78 respectively. The relatedness among the varieties assessed by Unweighted Pair Group Method with Arithmetic Mean (UPMGA) cluster analysis did not separate var. from the three cultivated varieties of . The result from the principal component analysis (PCA) further supports the genetic relatedness and groupings obtained from the cluster analysis. Overall, the study indicated that ISSR markers were effective in assessing the genetic relatedness and revealed genetic homogeneity of the four varieties. Our results, therefore, support the inclusion of var. as a variety of species.

Citing Articles

Genetic Variation Study of Several Romanian Pepper ( L.) Varieties Revealed by Molecular Markers and Whole Genome Resequencing.

Udriste A, Iordachescu M, Badulescu L Int J Mol Sci. 2024; 25(22).

PMID: 39595967 PMC: 11593692. DOI: 10.3390/ijms252211897.

Genetic Characterization of Some Saudi Arabia's Accessions from Using Physio-Biochemical Parameters, Molecular Markers, DNA Barcoding Analysis and Relative Gene Expression.

Safhi F, Alshamrani S, Jalal A, El-Moneim D, Alyamani A, Ibrahim A Genes (Basel). 2022; 13(11).

PMID: 36421774 PMC: 9690626. DOI: 10.3390/genes13112099.

fruits as functional ingredients with antimicrobial activity: an emphasis on mechanisms of action.

Romero-Luna H, Colina J, Guzman-Rodriguez L, Sierra-Carmona C, Farias-Campomanes A, Garcia-Pinilla S J Food Sci Technol. 2022; :1-11.

PMID: 36091639 PMC: 9441016. DOI: 10.1007/s13197-022-05578-y.

Basic concepts and methodologies of DNA marker systems in plant molecular breeding.

Amiteye S Heliyon. 2021; 7(10):e08093.

PMID: 34765757 PMC: 8569399. DOI: 10.1016/j.heliyon.2021.e08093.

DNA fingerprinting, fixation-index (Fst), and admixture mapping of selected Bambara groundnut (Vigna subterranea [L.] Verdc.) accessions using ISSR markers system.

Khan M, Rafii M, Ramlee S, Jusoh M, Mamun M, Halidu J Sci Rep. 2021; 11(1):14527.

PMID: 34267249 PMC: 8282841. DOI: 10.1038/s41598-021-93867-5.

References

1.

Chen S, Pang X, Song J, Shi L, Yao H, Han J . A renaissance in herbal medicine identification: from morphology to DNA. Biotechnol Adv. 2014; 32(7):1237-1244. DOI: 10.1016/j.biotechadv.2014.07.004. View

2.

Jia X, Wang T, Zhai M, Li Y, Guo Z . Genetic diversity and identification of Chinese-grown pecan using ISSR and SSR markers. Molecules. 2011; 16(12):10078-92. PMC: 6264290. DOI: 10.3390/molecules161210078. View

3.

Pfeiffer T, Roschanski A, Pannell J, Korbecka G, Schnittler M . Characterization of microsatellite loci and reliable genotyping in a polyploid plant, Mercurialis perennis (Euphorbiaceae). J Hered. 2011; 102(4):479-88. DOI: 10.1093/jhered/esr024. View

4.

Igwe D, Afiukwa C, Ubi B, Ogbu K, Bernard Ojuederie O, Ude G . Assessment of genetic diversity in Vigna unguiculata L. (Walp) accessions using inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) polymorphic markers. BMC Genet. 2017; 18(1):98. PMC: 5693802. DOI: 10.1186/s12863-017-0567-6. View

5.

Thul S, Darokar M, Shasany A, Khanuja S . Molecular profiling for genetic variability in Capsicum species based on ISSR and RAPD markers. Mol Biotechnol. 2011; 51(2):137-47. DOI: 10.1007/s12033-011-9446-y. View

6.

Carrizo Garcia C, Barfuss M, Sehr E, Barboza G, Samuel R, Moscone E . Phylogenetic relationships, diversification and expansion of chili peppers (Capsicum, Solanaceae). Ann Bot. 2016; 118(1):35-51. PMC: 4934398. DOI: 10.1093/aob/mcw079. View

7.

Ince A, Karaca M, Onus A . Genetic relationships within and between Capsicum species. Biochem Genet. 2009; 48(1-2):83-95. DOI: 10.1007/s10528-009-9297-4. View

8.

Patel H, Fougat R, Kumar S, Mistry J, Kumar M . Detection of genetic variation in Ocimum species using RAPD and ISSR markers. 3 Biotech. 2017; 5(5):697-707. PMC: 4569613. DOI: 10.1007/s13205-014-0269-y. View

9.

ANDERSON J, Churchill G, Autrique J, Tanksley S, Sorrells M . Optimizing parental selection for genetic linkage maps. Genome. 1993; 36(1):181-6. DOI: 10.1139/g93-024. View

10.

Olatunji T, Afolayan A . Comparative Quantitative Study on Phytochemical Contents and Antioxidant Activities of L. and L. ScientificWorldJournal. 2019; 2019:4705140. PMC: 6487110. DOI: 10.1155/2019/4705140. View

11.

Sunar S, Yildirim N, Sengul M, Agar G . Genetic diversity and relationships detected by ISSR and RAPD analysis among Aethionema species growing in Eastern Anatolia (Turkey). C R Biol. 2016; 339(3-4):147-51. DOI: 10.1016/j.crvi.2016.02.006. View

Evaluation of Genetic Relationship Among Varieties of L. and L. in West Africa Using ISSR Markers