Fabrication of KR-12 Peptide-containing Hyaluronic Acid Immobilized Fibrous Eggshell Membrane Effectively Kills Multi-drug-resistant Bacteria, Promotes Angiogenesis and Accelerates Re-epithelialization

Overview

Journal Int J Nanomedicine

Publisher Dove Medical Press

Specialty Biotechnology

Date 2019 Jun 14

PMID 31190796

Citations 19

Authors

Menglong Liu

Tengfei Liu

Xiaorong Zhang

Zhiwen Jian

Hesheng Xia

Jiacai Yang

Xiaohong Hu

Malcolm Xing

Gaoxing Luo

Jun Wu

Affiliations

Soon will be listed here.

Abstract

Designing a wound dressing that effectively prevents multi-drug-resistant bacterial infection and promotes angiogenesis and re-epithelialization is of great significance for wound management. In this study, a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA) was developed in an eco-friendly manner. The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide. Furthermore, the in vitrobiological results revealed that the composite membrane had excellent antibacterial activity against Gram-positive , methicillin-resistant (MRSA) and Gram-negative , and it could prevent MRSA biofilm formation on its surface. Additionally, it promoted the proliferation of keratinocytes and human umbilical vein endothelial cells and increased the secretion of VEGF. Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively. These results indicated that the composite membrane is a potential candidate of wound dressings.

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