Qingshen Buyang Formula Attenuates Renal Fibrosis in 5/6 Nephrectomized Rats Via Inhibiting EMT and Wnt/-Catenin Pathway

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2019 Jun 13

PMID 31186661

Citations 3

Authors

Xueqin Zhang

Jing Fang

Zhiqiang Chen

Bingwu Zhao

Su Wu

Yongmei Pan

Affiliations

Soon will be listed here.

Abstract

As renal fibrosis significantly contributes to various kinds of chronic kidney diseases, this study aimed to investigate the renal protective effects of Qingshen Buyang Formula against renal fibrosis on 5/6 nephrectomized rats, and its underlying mechanisms were explored. A total of 24 male Sprague-Dawley rats were randomly divided into sham operation group (Sham group), 5/6 nephrectomy group (5/6Nx group), and Qingshen Buyang Formula treatment group (QBF group). The intervention was intragastric administration for 12 weeks. In the end, the blood samples were collected to test renal functional parameters, urine proteins were measured, and the left kidneys were removed for histological studies, as well as mRNA and protein expression analysis. The results showed that Qingshen Buyang Formula significantly decreased BUN, Scr, and proteinuria in 5/6Nx rats. Meanwhile, it ameliorated the kidney injury and fibrosis, exemplified by the depressed expression of collagen I and fibronectin (FN), which are the main components of ECM. Furthermore, the process of EMT inhibited the Wnt/-catenin signaling pathway related genes, such as Wnt4, TCF4, -catenin, and p-GSK3. Collectively, the Qingshen Buyang Formula can improve renal function and attenuate renal fibrosis, and its underlying mechanisms may be related with inhibiting EMT and Wnt/-catenin signaling pathway.

Citing Articles

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Ma X, Qiang P, Chen G, Wang Z, Wang X, Xu Q Evid Based Complement Alternat Med. 2021; 2021:5533911.

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MicroRNA-27a targets Sfrp1 to induce renal fibrosis in diabetic nephropathy by activating Wnt/β-Catenin signalling.

Shi M, Tian P, Liu Z, Zhang F, Zhang Y, Qu L Biosci Rep. 2020; 40(6).

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