Structural and Biochemical Analysis of a Phosin from Streptomyces Chartreusis Reveals a Combined Polyphosphate- and Metal-binding Fold

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 2019 Jun 12

PMID 31183865

Citations 6

Authors

Sebastiaan Werten

Nils Hinnerk Rustmeier

Maximilian Gemmer

Marie-Joelle Virolle

Winfried Hinrichs

Affiliations

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Abstract

X-ray crystallographic analysis of a phosin (PptA) from Steptomyces chartreusis reveals a metal-associated, lozenge-shaped fold featuring a 5-10 Å wide, positively charged tunnel that traverses the protein core. Two distinct metal-binding sites were identified in which the predominant metal ion was Cu . In solution, PptA forms stable homodimers that bind with nanomolar affinity to polyphosphate, a stress-related biopolymer acting as a phosphate and energy reserve in conditions of nutrient depletion. A single protein dimer interacts with 14-15 consecutive phosphate moieties within the polymer. Our observations suggest that PptA plays a role in polyphosphate metabolism, mobilisation or sensing, possibly by acting in concert with polyphosphate kinase (Ppk). Like Ppk, phosins may influence antibiotic synthesis by streptomycetes.

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