Peroxisomes in Wild-type and Rosy Mutant Drosophila Melanogaster

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1987 Nov 1

PMID 3118368

Citations 15

Authors

M E Beard

E Holtzman

Affiliations

Soon will be listed here.

Abstract

This study shows that peroxisomes are abundant in the Malpighian tubule and gut of wild-type Oregon R Drosophila melanogaster and that the peroxisomal population of the rosy-506 eye-color mutant differs from that of the wild type. Catalase activity in wild-type flies is demonstrable in bodies of appearance and centrifugal behavior comparable to the peroxisomes of vertebrate tissues. Xanthine oxidase (xanthine:oxygen oxidoreductase, EC 1.1.3.22) activity of the Malpighian tubule of wild-type flies is demonstrable cytochemically in bodies like those containing catalase. The rosy-506 mutant flies, with a deletion in the structural gene for xanthine dehydrogenase (xanthine:NAD+ oxidoreductase, EC 1.1.1.204), lack cytochemically demonstrable peroxisomal xanthine oxidase activity. In addition, peroxisomes in the rosy-506 mutants show less intense cytochemical staining for catalase than those in wild-type flies, and biochemical assays indicate that catalase in the rosy mutant is much more accessible to substrate in the absence of detergent than in the wild type. Thus, the rosy-506 mutation appears to affect peroxisomes and may mimic aspects of the defects of peroxisomes in some human metabolic disorders.

Citing Articles

Rosy Beginnings: Studying Peroxisomes in .

Pridie C, Ueda K, Simmonds A Front Cell Dev Biol. 2020; 8:835.

PMID: 32984330 PMC: 7477296. DOI: 10.3389/fcell.2020.00835.

Bioinformatic prediction of critical genes and pathways involved in longevity in Drosophila melanogaster.

Li J, Duan D, Zhang J, Zhou Y, Qin X, Du G Mol Genet Genomics. 2019; 294(6):1463-1475.

PMID: 31327054 DOI: 10.1007/s00438-019-01589-1.

Distinct Roles for Peroxisomal Targeting Signal Receptors Pex5 and Pex7 in .

Di Cara F, Rachubinski R, Simmonds A Genetics. 2018; 211(1):141-149.

PMID: 30389805 PMC: 6325700. DOI: 10.1534/genetics.118.301628.

Dysfunctional peroxisomes compromise gut structure and host defense by increased cell death and Tor-dependent autophagy.

Di Cara F, Bulow M, Simmonds A, Rachubinski R Mol Biol Cell. 2018; 29(22):2766-2783.

PMID: 30188767 PMC: 6249834. DOI: 10.1091/mbc.E18-07-0434.

A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila.

Nagarkar-Jaiswal S, Lee P, Campbell M, Chen K, Anguiano-Zarate S, Gutierrez M Elife. 2015; 4.

PMID: 25824290 PMC: 4379497. DOI: 10.7554/eLife.05338.

References

Baudhuin P, BEAUFAY H, RAHMAN-LI Y, Sellinger O, WATTIAUX R, Jacques P . Tissue fractionation studies. 17. Intracellular distribution of monoamine oxidase, aspartate aminotransferase, alanine aminotransferase, D-amino acid oxidase and catalase in rat-liver tissue. Biochem J. 1964; 92(1):179-84. PMC: 1215456. DOI: 10.1042/bj0920179. View

Keller Jr E, Glassman E . A THIRD LOCUS (LXD) AFFECTING XANTHINE DEHYDROGENASE IN DROSOPHILA MELANOGASTER. Genetics. 1964; 49:663-8. PMC: 1210604. DOI: 10.1093/genetics/49.4.663. View

Stirpe F, Della Corte E . The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O). J Biol Chem. 1969; 244(14):3855-63. View

Hirai K . Light microscopic study of the peroxidatic activity of catalase in formaldehyde-fixed rat liver. J Histochem Cytochem. 1969; 17(9):585-90. DOI: 10.1177/17.9.585. View

Scott P, Visentin L, Allen J . The enzymatic characteristics of peroxisomes of amphibian and avian liver and kidney. Ann N Y Acad Sci. 1969; 168(2):244-64. DOI: 10.1111/j.1749-6632.1969.tb43113.x. View

Locke M, McMahon J . The origin and fate of microbodies in the fat body of an insect. J Cell Biol. 1971; 48(1):61-78. PMC: 2108215. DOI: 10.1083/jcb.48.1.61. View

Samis H, Baird M, Massie H . Renewal of catalase activity in Drosophila following treatment with 3-amino-1,2,4-triazole. J Insect Physiol. 1972; 18(5):991-1000. DOI: 10.1016/0022-1910(72)90036-4. View

NOVIKOFF A, Novikoff P, Davis C, Quintana N . Studies on microperoxisomes. II. A cytochemical method for light and electron microscopy. J Histochem Cytochem. 1972; 20(12):1006-23. DOI: 10.1177/20.12.1006. View

Romer F . Ultrastructural changes of the oenocytes of Gryllus bimaculatus DEG (Saltatoria, Insecta) during the moulting cycle. Cell Tissue Res. 1974; 151(1):27-46. DOI: 10.1007/BF00222032. View

10.

Lazarow P, DE DUVE C . The synthesis and turnover of rat liver peroxisomes. V. Intracellular pathway of catalase synthesis. J Cell Biol. 1973; 59(2 Pt 1):507-24. PMC: 2109097. DOI: 10.1083/jcb.59.2.507. View

11.

Briggs R, Drath D, Karnovsky M, Karnovsky M . Localization of NADH oxidase on the surface of human polymorphonuclear leukocytes by a new cytochemical method. J Cell Biol. 1975; 67(3):566-86. PMC: 2111669. DOI: 10.1083/jcb.67.3.566. View

12.

Larsen W . Cell remodeling in the fat body of an insect. Tissue Cell. 1976; 8(1):73-92. DOI: 10.1016/0040-8166(76)90021-5. View

13.

HANNA C, Hopkins T, Buck J . Peroxisomes of the firefly lantern. J Ultrastruct Res. 1976; 57(2):150-62. DOI: 10.1016/s0022-5320(76)80105-0. View

14.

Macintyre R, OBrien S . Interacting gene-enzyme systems in Drosophila. Annu Rev Genet. 1976; 10:281-318. DOI: 10.1146/annurev.ge.10.120176.001433. View

15.

Veenhuis M, Bonga S . The cytochemical demonstration of catalase and D-amino acid oxidase in the microbodies of teleost kidney cells. Histochem J. 1977; 9(2):171-81. DOI: 10.1007/BF01003629. View

16.

Goldenberg H . Organization of purpine degradation in the liver of a teleost (carp; Cyprinus carpio L.). A study of its subcellular distribution. Mol Cell Biochem. 1977; 16(1):17-21. DOI: 10.1007/BF01769834. View

17.

Arnold G, Liscum L, Holtzman E . Ultrastructural localization of D-amino acid oxidase in microperoxisomes of the rat nervous system. J Histochem Cytochem. 1979; 27(3):735-45. DOI: 10.1177/27.3.39097. View

18.

Hand A . Cytochemical detection of peroxisomal oxidases. J Histochem Cytochem. 1979; 27(10):1367-70. DOI: 10.1177/27.10.92494. View

19.

Arnold G, Holtzman E . Ultrastructural localization of alpha-OH acid oxidase in peroxisomes with the CeCl3 technique. J Histochem Cytochem. 1980; 28(9):1025-8. DOI: 10.1177/28.9.6997367. View

20.

Lazarow P, Robbi M, Fujiki Y, Wong L . Biogenesis of peroxisomal proteins in vivo and in vitro. Ann N Y Acad Sci. 1982; 386:285-300. DOI: 10.1111/j.1749-6632.1982.tb21423.x. View