Screening and Enzymatic Activity of High-efficiency Gellan Lyase Producing Bacteria PE1

Overview

Journal Bioengineered

Date 2019 Jun 12

PMID 31181994

Citations 1

Authors

Ang Li

Hangqi Luo

Tingting Hu

Jingyu Huang

Nafee-Ul Alam

Yuan Meng

Fenbin Meng

Nartey Linda Korkor

Xiufang Hu

Ou Li

Affiliations

Soon will be listed here.

Abstract

Gellan is a widely used microbial polysaccharide and one of the more effective ways to expand its application value would be to investigate the mechanism of gellan lyase and to produce gellan oligosaccharide. In this study, efficient gellan degrading bacteria were screened. One of the strains with high efficient gellan degradation capacity was labeled PE1. Through physiological and biochemical analysis of 16S rDNA, the species was identified as . The optimum conditions for enzymatic activity and how it was affected by metal ions were determined, and the results showed that the lyase activities were much higher than those of previously reported (about 20 times). The gellan degradation products were determined by thin-layer chromatography and the oligosaccharides were determined by high-efficiency liquid chromatography to analyze the action site of lyase. This study laid a solid foundation which elucidates the production and application of gellan oligosaccharides. ● High efficiency gellan lyase producing bacteria ● Optimization of reaction conditions for gellan degradation ● Oligosaccharides were detected by TLC and HPLC to speculate the lyase action sites.

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