PIN2 Polarity Establishment in in the Absence of an Intact Cytoskeleton

Overview

Journal Biomolecules

Publisher MDPI

Specialties Biochemistry
Molecular Biology

Date 2019 Jun 12

PMID 31181636

Citations 6

Authors

Matous Glanc

Matyas Fendrych

Jiri Friml

Affiliations

Soon will be listed here.

Abstract

Cell polarity is crucial for the coordinated development of all multicellular organisms. In plants, this is exemplified by the PIN-FORMED (PIN) efflux carriers of the phytohormone auxin: The polar subcellular localization of the PINs is instructive to the directional intercellular auxin transport, and thus to a plethora of auxin-regulated growth and developmental processes. Despite its importance, the regulation of PIN polar subcellular localization remains poorly understood. Here, we have employed advanced live-cell imaging techniques to study the roles of microtubules and actin microfilaments in the establishment of apical polar localization of PIN2 in the epidermis of the root meristem. We report that apical PIN2 polarity requires neither intact actin microfilaments nor microtubules, suggesting that the primary spatial cue for polar PIN distribution is likely independent of cytoskeleton-guided endomembrane trafficking.

Citing Articles

Divergence of trafficking and polarization mechanisms for PIN auxin transporters during land plant evolution.

Tang H, Lu K, Zhang Y, Cheng Y, Tu S, Friml J Plant Commun. 2023; 5(1):100669.

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An Arabidopsis mutant deficient in phosphatidylinositol-4-phosphate kinases ß1 and ß2 displays altered auxin-related responses in roots.

Starodubtseva A, Kalachova T, Retzer K, Jelinkova A, Dobrev P, Lacek J Sci Rep. 2022; 12(1):6947.

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Proteome-wide cellular thermal shift assay reveals unexpected cross-talk between brassinosteroid and auxin signaling.

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Bundling up the Role of the Actin Cytoskeleton in Primary Root Growth.

Garcia-Gonzalez J, van Gelderen K Front Plant Sci. 2022; 12:777119.

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Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana.

Li H, von Wangenheim D, Zhang X, Tan S, Darwish-Miranda N, Naramoto S New Phytol. 2020; 229(1):351-369.

PMID: 32810889 PMC: 7984064. DOI: 10.1111/nph.16887.

References

Smertenko A, Assaad F, Baluska F, Bezanilla M, Buschmann H, Drakakaki G . Plant Cytokinesis: Terminology for Structures and Processes. Trends Cell Biol. 2017; 27(12):885-894. DOI: 10.1016/j.tcb.2017.08.008. View

Brumos J, Robles L, Yun J, Vu T, Jackson S, Alonso J . Local Auxin Biosynthesis Is a Key Regulator of Plant Development. Dev Cell. 2018; 47(3):306-318.e5. DOI: 10.1016/j.devcel.2018.09.022. View

MARC , Granger , BRINCAT , Fisher , Kao , McCubbin . A GFP-MAP4 reporter gene for visualizing cortical microtubule rearrangements in living epidermal cells . Plant Cell. 1998; 10(11):1927-40. PMC: 143949. DOI: 10.1105/tpc.10.11.1927. View

von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benkova E, Friml J . Live tracking of moving samples in confocal microscopy for vertically grown roots. Elife. 2017; 6. PMC: 5498147. DOI: 10.7554/eLife.26792. View

Wang Y, Motes C, Mohamalawari D, Blancaflor E . Green fluorescent protein fusions to Arabidopsis fimbrin 1 for spatio-temporal imaging of F-actin dynamics in roots. Cell Motil Cytoskeleton. 2004; 59(2):79-93. DOI: 10.1002/cm.20024. View

Geldner N, Friml J, Stierhof Y, Jurgens G, Palme K . Auxin transport inhibitors block PIN1 cycling and vesicle trafficking. Nature. 2001; 413(6854):425-8. DOI: 10.1038/35096571. View

Paciorek T, Friml J . Auxin signaling. J Cell Sci. 2006; 119(Pt 7):1199-202. DOI: 10.1242/jcs.02910. View

Boutte Y, Crosnier M, Carraro N, Traas J, Satiat-Jeunemaitre B . The plasma membrane recycling pathway and cell polarity in plants: studies on PIN proteins. J Cell Sci. 2006; 119(Pt 7):1255-65. DOI: 10.1242/jcs.02847. View

Furutani M, Sakamoto N, Yoshida S, Kajiwara T, Robert H, Friml J . Polar-localized NPH3-like proteins regulate polarity and endocytosis of PIN-FORMED auxin efflux carriers. Development. 2011; 138(10):2069-78. DOI: 10.1242/dev.057745. View

10.

Kleine-Vehn J, Langowski L, Wisniewska J, Dhonukshe P, Brewer P, Friml J . Cellular and molecular requirements for polar PIN targeting and transcytosis in plants. Mol Plant. 2009; 1(6):1056-66. DOI: 10.1093/mp/ssn062. View

11.

Wisniewska J, Xu J, Seifertova D, Brewer P, Ruzicka K, Blilou I . Polar PIN localization directs auxin flow in plants. Science. 2006; 312(5775):883. DOI: 10.1126/science.1121356. View

12.

Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J . A Functional Study of AUXILIN-LIKE1 and 2, Two Putative Clathrin Uncoating Factors in Arabidopsis. Plant Cell. 2018; 30(3):700-716. PMC: 5894831. DOI: 10.1105/tpc.17.00785. View

13.

Clough S, Bent A . Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1999; 16(6):735-43. DOI: 10.1046/j.1365-313x.1998.00343.x. View

14.

Leyser O . Auxin Signaling. Plant Physiol. 2017; 176(1):465-479. PMC: 5761761. DOI: 10.1104/pp.17.00765. View

15.

Men S, Boutte Y, Ikeda Y, Li X, Palme K, Stierhof Y . Sterol-dependent endocytosis mediates post-cytokinetic acquisition of PIN2 auxin efflux carrier polarity. Nat Cell Biol. 2008; 10(2):237-44. DOI: 10.1038/ncb1686. View

16.

Dhonukshe P, Aniento F, Hwang I, Robinson D, Mravec J, Stierhof Y . Clathrin-mediated constitutive endocytosis of PIN auxin efflux carriers in Arabidopsis. Curr Biol. 2007; 17(6):520-7. DOI: 10.1016/j.cub.2007.01.052. View

17.

Glanc M, Fendrych M, Friml J . Mechanistic framework for cell-intrinsic re-establishment of PIN2 polarity after cell division. Nat Plants. 2018; 4(12):1082-1088. PMC: 6394824. DOI: 10.1038/s41477-018-0318-3. View

18.

Mravec J, Petrasek J, Li N, Boeren S, Karlova R, Kitakura S . Cell plate restricted association of DRP1A and PIN proteins is required for cell polarity establishment in Arabidopsis. Curr Biol. 2011; 21(12):1055-60. DOI: 10.1016/j.cub.2011.05.018. View

19.

Lankova M, Humpolickova J, Vosolsobe S, Cit Z, Lacek J, covan M . Determination of Dynamics of Plant Plasma Membrane Proteins with Fluorescence Recovery and Raster Image Correlation Spectroscopy. Microsc Microanal. 2016; 22(2):290-9. DOI: 10.1017/S1431927616000568. View

20.

Kitakura S, Vanneste S, Robert S, Lofke C, Teichmann T, Tanaka H . Clathrin mediates endocytosis and polar distribution of PIN auxin transporters in Arabidopsis. Plant Cell. 2011; 23(5):1920-31. PMC: 3123958. DOI: 10.1105/tpc.111.083030. View