1,25-Dihydroxyvitamin D3-mediated Inhibition of Human B Cell Differentiation

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1987 Sep 1

PMID 3117462

Citations 13

Authors

W C Chen

B Vayuvegula

S Gupta

Affiliations

Soon will be listed here.

Abstract

We have examined the mechanisms of 1,25-dihydroxyvitamin D3 (D3)-mediated inhibition of human B cell differentiation to immunoglobulin (Ig) secreting cells. B lymphocytes were purified from human tonsils and peripheral blood mononuclear cells. Mononuclear cells were separated into adherent cells and nonadherent cells. Cells were stimulated with Staphylococcus aureus Cowen I (SAC) or pokeweed mitogen (PWM) for 7 days and immunoglobulin production was measured by ELISA assay, 1,25-dihydroxyvitamin D3 was added at different times during cultures. 1,25-Dihydroxyvitamin D3, in a dose-dependent manner, inhibited both SAC and PWM-induced Ig production by mononuclear cells. The maximum inhibition was observed when 1,25-dihydroxyvitamin D3 was added at the beginning of culture, but inhibition could still be observed when 1,25-dihydroxyvitamin D3 was added on day 4 of cultures. The inhibitory effect of 1,25-dihydroxyvitamin D3 on Ig production was significantly greater on mononuclear cells than on nonadherent cells. Addition of in vitro purified IL-1 to nonadherent cells enhanced 1,25-dihydroxyvitamin D3-induced inhibition of Ig production. 1,25-Dihydroxyvitamin D3 also inhibited the expression of IL-2 receptors on B cells activated with SAC. 1,25-dihydroxyvitamin D3 did not inhibit Ig production by SAC preactivated B cell blasts in response to T cell supernatants. These data suggest that vitamin D3 inhibits Ig production by inhibiting IL-2 receptor expression on B cells and via its effect on adherent macrophages. Vitamin D3 does not influence the effect of differentiation factors on activated B cells that have already expressed growth/differentiation factor receptors.

Citing Articles

Impact of dietary vitamin D on immunoregulation and disease pathology in lupus-prone NZB/W F1 mice.

Kraemer A, Schafer A, Sprenger D, Sehnert B, Williams J, Luo A Front Immunol. 2022; 13:933191.

PMID: 36505422 PMC: 9730823. DOI: 10.3389/fimmu.2022.933191.

Effect of Vitamin D on Graft-versus-Host Disease.

Rodriguez-Gil A, Carrillo-Cruz E, Marrero-Cepeda C, Rodriguez G, Perez-Simon J Biomedicines. 2022; 10(5).

PMID: 35625724 PMC: 9138416. DOI: 10.3390/biomedicines10050987.

Low Levels of Vitamin D Promote Memory B Cells in Lupus.

Yamamoto E, Nguyen J, Liu J, Keller E, Campbell N, Zhang C Nutrients. 2020; 12(2).

PMID: 31978964 PMC: 7070834. DOI: 10.3390/nu12020291.

Vitamin D: Effect on Haematopoiesis and Immune System and Clinical Applications.

Medrano M, Carrillo-Cruz E, Montero I, Perez-Simon J Int J Mol Sci. 2018; 19(9).

PMID: 30205552 PMC: 6164750. DOI: 10.3390/ijms19092663.

Vitamin D in Human Immunodeficiency Virus Infection: Influence on Immunity and Disease.

Jimenez-Sousa M, Martinez I, Medrano L, Fernandez-Rodriguez A, Resino S Front Immunol. 2018; 9:458.

PMID: 29593721 PMC: 5857570. DOI: 10.3389/fimmu.2018.00458.

References

Haussler M, McCain T . Basic and clinical concepts related to vitamin D metabolism and action (first of two parts). N Engl J Med. 1977; 297(18):974-83. DOI: 10.1056/NEJM197711032971804. View

Miyaura C, Abe E, Kuribayashi T, Tanaka H, Konno K, Nishii Y . 1 alpha,25-Dihydroxyvitamin D3 induces differentiation of human myeloid leukemia cells. Biochem Biophys Res Commun. 1981; 102(3):937-43. DOI: 10.1016/0006-291x(81)91628-4. View

Tanaka H, Abe E, Miyaura C, Kuribayashi T, Konno K, Nishii Y . 1 alpha,25-Dihydroxycholecalciferol and a human myeloid leukaemia cell line (HL-60). Biochem J. 1982; 204(3):713-9. PMC: 1158411. DOI: 10.1042/bj2040713. View

Bhalla A, Amento E, Clemens T, Holick M, Krane S . Specific high-affinity receptors for 1,25-dihydroxyvitamin D3 in human peripheral blood mononuclear cells: presence in monocytes and induction in T lymphocytes following activation. J Clin Endocrinol Metab. 1983; 57(6):1308-10. DOI: 10.1210/jcem-57-6-1308. View

Matsui T, Nakao Y, Kobayashi N, Kishihara M, Ishizuka S, Watanabe S . Phenotypic differentiation-linked growth inhibition in human leukemia cells by active vitamin D3 analogues. Int J Cancer. 1984; 33(2):193-202. DOI: 10.1002/ijc.2910330207. View

Amento E, Bhalla A, Kurnick J, Kradin R, Clemens T, Holick S . 1 alpha,25-dihydroxyvitamin D3 induces maturation of the human monocyte cell line U937, and, in association with a factor from human T lymphocytes, augments production of the monokine, mononuclear cell factor. J Clin Invest. 1984; 73(3):731-9. PMC: 425075. DOI: 10.1172/JCI111266. View

Tsoukas C, Provvedini D, Manolagas S . 1,25-dihydroxyvitamin D3: a novel immunoregulatory hormone. Science. 1984; 224(4656):1438-40. DOI: 10.1126/science.6427926. View

Lemire J, Adams J, Sakai R, Jordan S . 1 alpha,25-dihydroxyvitamin D3 suppresses proliferation and immunoglobulin production by normal human peripheral blood mononuclear cells. J Clin Invest. 1984; 74(2):657-61. PMC: 370520. DOI: 10.1172/JCI111465. View

Bhalla A, Amento E, Serog B, Glimcher L . 1,25-Dihydroxyvitamin D3 inhibits antigen-induced T cell activation. J Immunol. 1984; 133(4):1748-54. View

10.

Ravid A, Koren R, Novogrodsky A, Liberman U . 1,25-Dihydroxyvitamin D3 inhibits selectively the mitogenic stimulation of mouse medullary thymocytes. Biochem Biophys Res Commun. 1984; 123(1):163-9. DOI: 10.1016/0006-291x(84)90394-2. View

11.

Rigby W, Stacy T, Fanger M . Inhibition of T lymphocyte mitogenesis by 1,25-dihydroxyvitamin D3 (calcitriol). J Clin Invest. 1984; 74(4):1451-5. PMC: 425314. DOI: 10.1172/JCI111557. View

12.

Lemire J, Adams J, Kermani-Arab V, Bakke A, Sakai R, Jordan S . 1,25-Dihydroxyvitamin D3 suppresses human T helper/inducer lymphocyte activity in vitro. J Immunol. 1985; 134(5):3032-5. View

13.

Komoriya K, Nagata I, Tsuchimoto M, Kunisawa K, Takeshita T, Naruchi T . 1,25-Dihydroxyvitamin D3 and 1,24-dihydroxyvitamin D3 suppress in vitro antibody response to T cell-dependent antigen. Biochem Biophys Res Commun. 1985; 127(3):753-8. DOI: 10.1016/s0006-291x(85)80007-3. View

14.

Manolagas S, Provvedini D, Tsoukas C . Interactions of 1,25-dihydroxyvitamin D3 and the immune system. Mol Cell Endocrinol. 1985; 43(2-3):113-22. DOI: 10.1016/0303-7207(85)90074-7. View

15.

Rook G, Steele J, Fraher L, Barker S, Karmali R, ORiordan J . Vitamin D3, gamma interferon, and control of proliferation of Mycobacterium tuberculosis by human monocytes. Immunology. 1986; 57(1):159-63. PMC: 1453883. View

16.

Gray T, Cohen M . Vitamin D, phagocyte differentiation and immune function. Surv Immunol Res. 1985; 4(3):200-12. DOI: 10.1007/BF02918673. View

17.

Iho S, Kura F, Sugiyama H, Takahashi T, Hoshino T . The role of monocytes in the suppression of PHA-induced proliferation and IL 2 production of human mononuclear cells by 1,25-dihydroxyvitamin D3. Immunol Lett. 1985; 11(5-6):331-6. DOI: 10.1016/0165-2478(85)90116-6. View

18.

Polla B, Healy A, Amento E, Krane S . 1,25-Dihydroxyvitamin D3 maintains adherence of human monocytes and protects them from thermal injury. J Clin Invest. 1986; 77(4):1332-9. PMC: 424489. DOI: 10.1172/JCI112438. View

19.

Iho S, Takahashi T, Kura F, Sugiyama H, Hoshino T . The effect of 1,25-dihydroxyvitamin D3 on in vitro immunoglobulin production in human B cells. J Immunol. 1986; 136(12):4427-31. View

20.

Munker R, Norman A, Koeffler H . Vitamin D compounds. Effect on clonal proliferation and differentiation of human myeloid cells. J Clin Invest. 1986; 78(2):424-30. PMC: 423571. DOI: 10.1172/JCI112593. View