The Systemic Effect of PEG-nGO-induced Oxidative Stress in Vivo in a Rodent Model

Overview

Journal Beilstein J Nanotechnol

Specialty Biotechnology

Date 2019 Jun 6

PMID 31165017

Citations 31

Authors

Qura Tul Ain

Samina Hyder Haq

Abeer Alshammari

Moudhi Abdullah Al-Mutlaq

Muhammad Naeem Anjum

Affiliations

Soon will be listed here.

Abstract

Oxidative stress (OS) plays an important role in the pathology of certain human diseases. Scientists have developed great interest regarding the determination of oxidative stress caused after the administration of nano-graphene composites (PEG-nGO). Graphene oxide sheets (GOS) were synthesized via a modified Hummer's method and were characterized by X-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV), and transmission electron microscopy (TEM). The method of Zhang was adopted for cracking of GOS. Then nano-graphene oxide was PEGylated with polyethylene glycol (PEG). PEGylation of nGO was confirmed by Fourier-transform infrared spectroscopy (FTIR), UV spectroscopy and TEM. The average size distribution of nGO and PEG-nGO was determined by using dynamic light scattering (DLS). Subsequently, an in vivo study measuring a marker for oxidative stress, namely lipid peroxides, as well as antioxidant agents, including catalase, superoxide dismutase, glutathione, and glutathione S-transferase was conducted. A comparison at different intervals of time after the administration of a dose (5 mg/kg) of PEG-nGO was carried out. An increase in free radicals and a decrease in free radical scavenging enzymes in organs were observed. Our results indicated that the treatment with PEG-nGO caused an increased OS to the organs in the first few hours of treatment. However, the liver completely recovered from the OS after 4 h. Brain, heart and kidneys showed an increased OS even after 4 h. In conclusion increased OS induced by PEG-nGO could be detrimental to brain, heart and kidneys.

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References

Boyland E, Chasseaud L . The role of glutathione and glutathione S-transferases in mercapturic acid biosynthesis. Adv Enzymol Relat Areas Mol Biol. 1969; 32:173-219. DOI: 10.1002/9780470122778.ch5. View

Duan G, Zhang Y, Luan B, Weber J, Zhou R, Yang Z . Graphene-Induced Pore Formation on Cell Membranes. Sci Rep. 2017; 7:42767. PMC: 5317030. DOI: 10.1038/srep42767. View

Gies V, Zou S . Systematic toxicity investigation of graphene oxide: evaluation of assay selection, cell type, exposure period and flake size. Toxicol Res (Camb). 2018; 7(1):93-101. PMC: 6061886. DOI: 10.1039/c7tx00278e. View

Singh U, Jialal I . Oxidative stress and atherosclerosis. Pathophysiology. 2006; 13(3):129-42. DOI: 10.1016/j.pathophys.2006.05.002. View

Li B, Zhang X, Yang J, Zhang Y, Li W, Fan C . Influence of polyethylene glycol coating on biodistribution and toxicity of nanoscale graphene oxide in mice after intravenous injection. Int J Nanomedicine. 2014; 9:4697-707. PMC: 4207078. DOI: 10.2147/IJN.S66591. View

Tadyszak K, Wychowaniec J, Litowczenko J . Biomedical Applications of Graphene-Based Structures. Nanomaterials (Basel). 2018; 8(11). PMC: 6267346. DOI: 10.3390/nano8110944. View

Shen H, Liu M, He H, Zhang L, Huang J, Chong Y . PEGylated graphene oxide-mediated protein delivery for cell function regulation. ACS Appl Mater Interfaces. 2012; 4(11):6317-23. DOI: 10.1021/am3019367. View

Zhang L, Xia J, Zhao Q, Liu L, Zhang Z . Functional graphene oxide as a nanocarrier for controlled loading and targeted delivery of mixed anticancer drugs. Small. 2009; 6(4):537-44. DOI: 10.1002/smll.200901680. View

Dubovskiy I, Martemyanov V, Vorontsova Y, Rantala M, Gryzanova E, Glupov V . Effect of bacterial infection on antioxidant activity and lipid peroxidation in the midgut of Galleria mellonella L. larvae (Lepidoptera, Pyralidae). Comp Biochem Physiol C Toxicol Pharmacol. 2008; 148(1):1-5. DOI: 10.1016/j.cbpc.2008.02.003. View

10.

Winrow V, Winyard P, Morris C, Blake D . Free radicals in inflammation: second messengers and mediators of tissue destruction. Br Med Bull. 1993; 49(3):506-22. DOI: 10.1093/oxfordjournals.bmb.a072627. View

11.

Zhang W, Guo Z, Huang D, Liu Z, Guo X, Zhong H . Synergistic effect of chemo-photothermal therapy using PEGylated graphene oxide. Biomaterials. 2011; 32(33):8555-61. DOI: 10.1016/j.biomaterials.2011.07.071. View

12.

Yang K, Feng L, Shi X, Liu Z . Nano-graphene in biomedicine: theranostic applications. Chem Soc Rev. 2012; 42(2):530-47. DOI: 10.1039/c2cs35342c. View

13.

Langer R . Drug delivery. Drugs on target. Science. 2001; 293(5527):58-9. DOI: 10.1126/science.1063273. View

14.

Kakkar P, Das B, Viswanathan P . A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys. 1984; 21(2):130-2. View

15.

Liu S, Zeng T, Hofmann M, Burcombe E, Wei J, Jiang R . Antibacterial activity of graphite, graphite oxide, graphene oxide, and reduced graphene oxide: membrane and oxidative stress. ACS Nano. 2011; 5(9):6971-80. DOI: 10.1021/nn202451x. View

16.

Akhavan O, Ghaderi E . Toxicity of graphene and graphene oxide nanowalls against bacteria. ACS Nano. 2010; 4(10):5731-6. DOI: 10.1021/nn101390x. View

17.

Feng L, Yang X, Shi X, Tan X, Peng R, Wang J . Polyethylene glycol and polyethylenimine dual-functionalized nano-graphene oxide for photothermally enhanced gene delivery. Small. 2013; 9(11):1989-97. DOI: 10.1002/smll.201202538. View

18.

Zhang L, Xu B, Wang X . Cholesterol Extraction from Cell Membrane by Graphene Nanosheets: A Computational Study. J Phys Chem B. 2016; 120(5):957-64. DOI: 10.1021/acs.jpcb.5b10330. View

19.

Anreddy R, Yellu N, Devarakonda K . Oxidative biomarkers to assess the nanoparticle-induced oxidative stress. Methods Mol Biol. 2013; 1028:205-19. DOI: 10.1007/978-1-62703-475-3_13. View

20.

Simonian N, Coyle J . Oxidative stress in neurodegenerative diseases. Annu Rev Pharmacol Toxicol. 1996; 36:83-106. DOI: 10.1146/annurev.pa.36.040196.000503. View