The Poly(C) Motif in the Proximal Promoter Region of the D Site-Binding Protein Gene () Drives Its High-Amplitude Oscillation

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 2019 Jun 5

PMID 31160492

Citations 4

Authors

Paul Kwangho Kwon

Hyo-Min Kim

Sung Wook Kim

Byunghee Kang

Hee Yi

Hyun-Ok Ku

Tae-Young Roh

Kyong-Tai Kim

Affiliations

Soon will be listed here.

Abstract

The D site-binding protein (Dbp) supports the rhythmic transcription of downstream genes, in part by displaying high-amplitude cycling of its own transcripts compared to other circadian-clock genes. However, the underlying mechanism remains elusive. Here, we demonstrated that the poly(C) motif within the proximal promoter, in addition to an E-box element, provoked transcriptional activation. Furthermore, we generated a cell line with poly(C) deleted to demonstrate the endogenous effect of the poly(C) motif within the promoter. We investigated whether RNA polymerase 2 (Pol2) recruitment on the promoter was decreased in the cell line with poly(C) deleted. Next, assay for transposase-accessible chromatin (ATAC)-quantitative PCR (qPCR) showed that the poly(C) motif induced greater chromatin accessibility within the region of the promoter. Finally, we determined that the oscillation amplitude of endogenous mRNA of the cell line with poly(C) deleted was decreased, which affected the oscillation of other clock genes that are controlled by Taken together, our results provide new insights into the function of the poly(C) motif as a novel -acting element of , along with its significance in the regulation of circadian rhythms.

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