, Encoding A NAC Transcription Factor, Is Involved in Leaf Senescence in Grapevine

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2019 Jun 2

PMID 31151316

Citations 13

Authors

Ziguo Zhu

Guirong Li

Chaohui Yan

Li Liu

Qingtian Zhang

Zhen Han

Bo Li

Affiliations

Soon will be listed here.

Abstract

The NAC (for NAM, ATAF1,2, and CUC2) proteins family are plant-specific transcription factors, which play important roles in leaf development and response to environmental stresses. In this study, an NAC gene, , isolated from grapevine L. "Yatomi Rose", was shown to be involved in leaf senescence. The quantity of transcripts decreased with advancing leaf senescence in grapevine. Overexpressing the gene in tobacco plants significantly delayed leaf senescence with respect to chlorophyll concentration, potential quantum efficiency of photosystem II (Fv/Fm), and ion leakage. Moreover, exogenous abscisic acid (ABA) markedly reduced the expression of , and the ABA and salicylic acid (SA) concentration was lower in the -overexpressing transgenic plants than in the wild-type plants. The transgenic plants exhibited reduced sensitivity to ABA-induced senescence but no significant change in the sensitivity to jasmonic acid-, SA- or ethylene-induced senescence. Transcriptomic analysis and RNA expression studies also indicated that the transcript abundance of genes associated with ABA biosynthesis and regulation, including 9--epoxycarotenoid dioxygenase (), , zeaxanthin epoxidase1 (), ABA DEFICIENT2 (), , and ABA INSENSITIVE 2 (), was markedly reduced in the -overexpressing plants. These results suggested that plays a role as a negative regulator of leaf senescence by regulating ABA synthesis.

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