EFFECT OF PROGESTERONE ON SURVIVAL, GROWTH AND MATURATION OF FOLLICLE DERIVED FROM MOUSE POLYCYSTIC OVARY

Overview

Journal Acta Endocrinol (Buchar)

Specialty Endocrinology

Date 2019 Jun 1

PMID 31149276

Authors

A Bazdar

M Azadbakht

Affiliations

Soon will be listed here.

Abstract

Context: Polycystic ovarian syndrome is the most common endocrine disorder in women of reproductive age.

Objective: The purpose of the present study is to investigate the role of progesterone on survival, growth and maturation of follicles and the maturation of oocytes derived from mouse polycystic ovary.

Subjects And Methods: To induce polycystic ovary (PCO) female NMRI mice 21 days old were injected daily with testosterone enanthate 1 mg/100g body weight dissolved in sesame oil for 4 weeks (PCO group), while non-PCO group were injected only with vehicle. Follicles were derived from both groups and cultured in MEM-α medium either with progesterone or without progesterone. Sizes of follicles were measured in days 1, 3, 6, 9 and 12. After 12 days follicles were transferred to mature medium, follicles from two groups were cultured for 24 and 48 h and the maturation of oocytes was assessed.

Results: In PCO groups with progesterone, survival and growth of follicles, significantly increased as compared with PCO groups without progesterone (p<0.05). The maturation rate in PCO group with progesterone was significantly higher than in those not treated by progesterone (p<0.05).

Conclusions: This study demonstrated that progesterone can improve survival, growth and maturation of follicles derived from mouse polycystic ovary.

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