Scribble, Erbin, and Lano Redundantly Regulate Epithelial Polarity and Apical Adhesion Complex

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2019 Jun 1

PMID 31147384

Citations 28

Authors

Jongho Choi

Regina B Troyanovsky

Indrajyoti Indra

Brian J Mitchell

Sergey M Troyanovsky

Affiliations

Soon will be listed here.

Abstract

The basolateral protein Scribble (Scrib), a member of the LAP protein family, is essential for epithelial apicobasal polarity (ABP) in However, a conserved function for this protein in mammals is unclear. Here we show that the crucial role for Scrib in ABP has remained obscure due to the compensatory function of two other LAP proteins, Erbin and Lano. A combined Scrib/Erbin/Lano knockout disorganizes the cell-cell junctions and the cytoskeleton. It also results in mislocalization of several apical (Par6, aPKC, and Pals1) and basolateral (Llgl1 and Llgl2) identity proteins. These defects can be rescued by the conserved "LU" region of these LAP proteins. Structure-function analysis of this region determined that the so-called LAPSDb domain is essential for basolateral targeting of these proteins, while the LAPSDa domain is essential for supporting the membrane basolateral identity and binding to Llgl. In contrast to the key role in , mislocalization of Llgl proteins does not appear to be critical in the ABP phenotype.

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