The Biochemical Activities of the Pif1 Helicase Are Regulated by Its N-Terminal Domain

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2019 May 31

PMID 31142053

Citations 14

Authors

David G Nickens

Christopher W Sausen

Matthew L Bochman

Affiliations

Soon will be listed here.

Abstract

Pif1 family helicases represent a highly conserved class of enzymes involved in multiple aspects of genome maintenance. Many Pif1 helicases are multi-domain proteins, but the functions of their non-helicase domains are poorly understood. Here, we characterized how the N-terminal domain (NTD) of the Pif1 helicase affects its functions both in vivo and in vitro. Removal of the Pif1 NTD alleviated the toxicity associated with Pif1 overexpression in yeast. Biochemically, the N-terminally truncated Pif1 (Pif1ΔN) retained in vitro DNA binding, DNA unwinding, and telomerase regulation activities, but these activities differed markedly from those displayed by full-length recombinant Pif1. However, Pif1ΔN was still able to synergize with the Hrq1 helicase to inhibit telomerase activity in vitro, similar to full-length Pif1. These data impact our understanding of Pif1 helicase evolution and the roles of these enzymes in the maintenance of genome integrity.

Citing Articles

Alternative translation initiation by ribosomal leaky scanning produces multiple isoforms of the Pif1 helicase.

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Cdc13 exhibits dynamic DNA strand exchange in the presence of telomeric DNA.

Nickens D, Feng Z, Shen J, Gray S, Simmons 3rd R, Niu H Nucleic Acids Res. 2024; 52(11):6317-6332.

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The functional significance of the RPA- and PCNA-dependent recruitment of Pif1 to DNA.

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Bulk phase biochemistry of PIF1 and RecQ4 family helicases.

Rajapaksha P, Simmons 3rd R, Gray S, Sun D, Nguyen P, Nickens D Methods Enzymol. 2022; 673:169-190.

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Role and Regulation of Pif1 Family Helicases at the Replication Fork.

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