Comprehensive Analysis of Key Genes and MicroRNAs in Radioresistant Nasopharyngeal Carcinoma

Overview

Journal BMC Med Genomics

Publisher Biomed Central

Specialty Genetics

Date 2019 May 30

PMID 31138194

Citations 14

Authors

Ya Guo

Yang Zhang

Shu Juan Zhang

Yi Nan Ma

Yun He

Affiliations

Soon will be listed here.

Abstract

Background: Radioresistance is one of the main obstacle limiting the therapeutic efficacy and prognosis of patients, the molecular mechanisms of radioresistance is still unclear. The purpose of this study was to identify the key genes and miRNAs and to explore their potential molecular mechanisms in radioresistant nasopharyngeal carcinoma.

Methods: In this study, we analysis the differentially expressed genes and microRNA based on the database of GSE48501 and GSE48502, and then employed bioinformatics to analyze the pathways and GO terms in which DEGs and DEMS target genes are involved. Moreover, Construction of protein-protein interaction network and identification of hub genes. Finally, analyzed the biological networks for validated target gene of hub miRNAs.

Results: A total of 373 differentially expressed genes (DEGs) and 14 differentially expressed microRNAs (DEMs) were screened out. The up-regulated gene JUN was overlap both in DEGs and publicly available studies, which was potentially targeted by three miRNAs, including hsa-miR-203, hsa-miR-24 and hsa-miR-31. Moreover, Pathway analysis showed that both up-regulated gene and DEMs target genes were enriched in TGF-beta signaling pathway, Hepatitis B, Pathways in cancer and p53 signaling pathway. Finally, we further constructed protein-protein interaction network (PPI) of DEGs and analyzed the biological networks for above mentioned common miRNAs, the result indicated that JUN was a core hub gene in PPI network, hsa-miR-24 and its target gene were significantly enriched in P53 signaling pathway.

Conclusions: These results might provide new clues to improve the radiosensitivity of Nasopharyngeal Carcinoma.

Citing Articles

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Homeobox B2 promotes malignant behavior and contributes to the radioresistance of nasopharyngeal carcinoma by regulating forkhead box protein O1.

Chen J, Mao M, Ma Z, Liu J, Jiang M, Chen G Int J Med Sci. 2024; 21(5):837-847.

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The Highly Expressed IFIT1 in Nasopharyngeal Carcinoma Enhances Proliferation, Migration, and Invasion of Nasopharyngeal Carcinoma Cells.

Wu X, Lin L, Zhou F, Yu S, Chen M, Wang S Mol Biotechnol. 2022; 64(6):621-636.

PMID: 35038119 DOI: 10.1007/s12033-021-00439-z.

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