Protective Effect of Echinacea Purpurea (Immulant) Against Cisplatin-induced Immunotoxicity in Rats

Overview

Journal Daru

Specialty Pharmacology

Date 2019 May 29

PMID 31134491

Citations 10

Authors

Abdelazeem Ali Khalaf

Shaymaa Hussein

Adel Fathy Tohamy

Sherif Marouf

Hanan Dawood Yassa

Amr Reda Zaki

Anupam Bishayee

Affiliations

Soon will be listed here.

Abstract

Purpose: Cisplatin, one of the most effective anticancer drugs, is known to cause undesirable adverse effects, including immunotoxicity. Echinacea purpurea is an important medicinal plant with immunostimulatory and anti-inflammatory activities. We have investigated the protective effect of an herbal formulation (Immulant) containing E. purpurea extract against cisplatin-induced immunotoxicity in rats.

Methods: Forty mature albino rats were randomized into four groups (10 rats/group). Control (group 1) animals were subjected to intraperitoneal (i.p.) injection of saline solution (0.2 ml) once every 3 days. Group 2 animals received cisplatin (3.5 mg/kg, i.p.) once every 3 days for successive 2 weeks. Group 3 rats received oral Immulant (150 mg/kg) once daily for 2 weeks. Group 4 animals received oral Immulant treatment as in group 3 in addition to cisplatin as in group 2. Serum level of total protein and albumin, total and differential leukocytic count, phagocytic activity of monocytes, humoral activity and splenic histopathology and immunohistochemistry were used as diagnostic markers of immunotoxicity.

Results: Cisplatin induced marked inhibition of cellular immunity as exhibited by significant decrease of leukocytic count, lymphocyte percentage and phagocytic activity with marked increase in neutrophil percentage. Humoral immunity represented by marked inhibition in total protein and γ-globulin concentration and significant inhibition in antibody titer against Mycoplasma gallisepticum were recorded. Histopathological and immunohistochemical observation of the spleen of cisplatin-treated rats revealed obvious pathological findings of marked depletion and degeneration of lymphoid tissue. Co-oral administration of Immulant resulted in substantial improvement of various immunotoxicological indices compared to cisplatin control.

Conclusion: The herbal medicine Immulant is an immunostimulant which could be used to treat the immunotoxic effects of cisplatin. Graphical abstract Cisplatin (CP) is a highly effective antineoplastic DNA alkylating agent. CP induces free radical production causing an oxidative damage.Cisplatin induced marked inhibition in cellular and humoral immunityEchinacea purpurea (Immulant) is a powerful anticytotoxic agent against cisplatin toxicity.

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References

Jordan P, Carmo-Fonseca M . Molecular mechanisms involved in cisplatin cytotoxicity. Cell Mol Life Sci. 2000; 57(8-9):1229-35. PMC: 11147023. DOI: 10.1007/pl00000762. View

Kim L, Waters R, Burkholder P . Immunological activity of larch arabinogalactan and Echinacea: a preliminary, randomized, double-blind, placebo-controlled trial. Altern Med Rev. 2002; 7(2):138-49. View

Mantovani M, Bellini M, Angeli J, Oliveira R, Silva A, Ribeiro L . beta-Glucans in promoting health: prevention against mutation and cancer. Mutat Res. 2007; 658(3):154-61. DOI: 10.1016/j.mrrev.2007.07.002. View

Wijermans P, Gerrits W, Haak H . Severe immunodeficiency in patients treated with fludarabine monophosphate. Eur J Haematol. 1993; 50(5):292-6. DOI: 10.1111/j.1600-0609.1993.tb00165.x. View

Hassan I, Chibber S, Naseem I . Ameliorative effect of riboflavin on the cisplatin induced nephrotoxicity and hepatotoxicity under photoillumination. Food Chem Toxicol. 2010; 48(8-9):2052-8. DOI: 10.1016/j.fct.2010.05.004. View

Markovic S, Djacic D, Cvetkovic D, Obradovic A, Zizic J, Ognjanovic B . Effects of acute in vivo cisplatin and selenium treatment on hematological and oxidative stress parameters in red blood cells of rats. Biol Trace Elem Res. 2010; 142(3):660-70. DOI: 10.1007/s12011-010-8788-9. View

Ramos-Vara J . Technical aspects of immunohistochemistry. Vet Pathol. 2005; 42(4):405-26. DOI: 10.1354/vp.42-4-405. View

Descotes J . Methods of evaluating immunotoxicity. Expert Opin Drug Metab Toxicol. 2006; 2(2):249-59. DOI: 10.1517/17425255.2.2.249. View

Pearse G, Pietersma A, Cunliffe J, Foster J, Turton J, Derbyshire N . Time-course study of the immunotoxic effects of the anticancer drug chlorambucil in the rat. Toxicol Pathol. 2009; 37(7):887-901. DOI: 10.1177/0192623309347907. View

10.

Menezes S, Bozza P, Castro Faria Neto H, Laranjeira A, Negri E, Capelozzi V . Pulmonary and extrapulmonary acute lung injury: inflammatory and ultrastructural analyses. J Appl Physiol (1985). 2005; 98(5):1777-83. DOI: 10.1152/japplphysiol.01182.2004. View

11.

Zink T, Chaffin J . Herbal 'health' products: what family physicians need to know. Am Fam Physician. 1998; 58(5):1133-40. View

12.

Kuper C, Harleman J, Vos J . Histopathologic approaches to detect changes indicative of immunotoxicity. Toxicol Pathol. 2000; 28(3):454-66. DOI: 10.1177/019262330002800317. View

13.

Zandvoort A, Lodewijk M, Klok P, Dammers P, Kroese F, Timens W . Slow recovery of follicular B cells and marginal zone B cells after chemotherapy: implications for humoral immunity. Clin Exp Immunol. 2001; 124(2):172-9. PMC: 1906061. DOI: 10.1046/j.1365-2249.2001.01530.x. View

14.

Tuorkey M . Cancer Therapy with Phytochemicals: Present and Future Perspectives. Biomed Environ Sci. 2015; 28(11):808-19. DOI: 10.3967/bes2015.112. View

15.

Barrett B . Medicinal properties of Echinacea: a critical review. Phytomedicine. 2003; 10(1):66-86. DOI: 10.1078/094471103321648692. View

16.

HEGAB S, Al-Mutawa S . Immunopathogenesis of Behçet's disease. Clin Immunol. 2000; 96(3):174-86. DOI: 10.1006/clim.2000.4901. View

17.

Jilek P, Dvorakova J, Tureckova J, Prochazkova J . The effect of platinum cytostatics on delayed-type hypersensitivity in mice. Neoplasma. 1989; 36(6):659-65. View

18.

Matthias A, Banbury L, Bone K, Leach D, Lehmann R . Echinacea alkylamides modulate induced immune responses in T-cells. Fitoterapia. 2007; 79(1):53-8. DOI: 10.1016/j.fitote.2007.07.012. View

19.

Liang B, Wang S, Ye Y, Yang X, Wang Y, Qu J . Impact of postoperative omega-3 fatty acid-supplemented parenteral nutrition on clinical outcomes and immunomodulations in colorectal cancer patients. World J Gastroenterol. 2008; 14(15):2434-9. PMC: 2705104. DOI: 10.3748/wjg.14.2434. View

20.

Li X, Schluesener H . Therapeutic effects of cisplatin on rat experimental autoimmune encephalomyelitis. Arch Immunol Ther Exp (Warsz). 2006; 54(1):51-3. DOI: 10.1007/s00005-006-0005-3. View