Characterization of a Broad-based Mosquito Yeast Interfering RNA Larvicide with a Conserved Target Site in Mosquito Semaphorin-1a Genes

Overview

Journal Parasit Vectors

Publisher Biomed Central

Specialties Parasitology
Tropical Medicine

Date 2019 May 24

PMID 31118082

Citations 25

Authors

Keshava Mysore

Ping Li

Chien-Wei Wang

Limb K Hapairai

Nicholas D Scheel

Jacob S Realey

Longhua Sun

David W Severson

Na Wei

Molly Duman-Scheel

Affiliations

Soon will be listed here.

Abstract

Background: RNA interference (RNAi), which has facilitated functional characterization of mosquito neural development genes such as the axon guidance regulator semaphorin-1a (sema1a), could one day be applied as a new means of vector control. Saccharomyces cerevisiae (baker's yeast) may represent an effective interfering RNA expression system that could be used directly for delivery of RNA pesticides to mosquito larvae. Here we describe characterization of a yeast larvicide developed through bioengineering of S. cerevisiae to express a short hairpin RNA (shRNA) targeting a conserved site in mosquito sema1a genes.

Results: Experiments conducted on Aedes aegypti larvae demonstrated that the yeast larvicide effectively silences sema1a expression, generates severe neural defects, and induces high levels of larval mortality in laboratory, simulated-field, and semi-field experiments. The larvicide was also found to induce high levels of Aedes albopictus, Anopheles gambiae and Culex quinquefasciatus mortality.

Conclusions: The results of these studies indicate that use of yeast interfering RNA larvicides targeting mosquito sema1a genes may represent a new biorational tool for mosquito control.

Citing Articles

Development of a controlled-release mosquito RNAi yeast larvicide suitable for the sustained control of large water storage containers.

Mysore K, Oxley J, Duckham C, Castilla-Gutierrez C, Stewart A, Winter N Sci Rep. 2024; 14(1):30186.

PMID: 39632972 PMC: 11618383. DOI: 10.1038/s41598-024-81800-5.

Challenges of Robust RNAi-Mediated Gene Silencing in Mosquitoes.

Figueiredo Prates L, Fiebig J, Schlosser H, Liapi E, Rehling T, Lutrat C Int J Mol Sci. 2024; 25(10).

PMID: 38791257 PMC: 11121262. DOI: 10.3390/ijms25105218.

Interkingdom interactions shape the fungal microbiome of mosquitoes.

Hegde S, Khanipov K, Hornett E, Nilyanimit P, Pimenova M, Saldana M Anim Microbiome. 2024; 6(1):11.

PMID: 38454530 PMC: 10921588. DOI: 10.1186/s42523-024-00298-4.

Outlook on RNAi-Based Strategies for Controlling Biting Midges.

Osborne C, Cohnstaedt L, Silver K Pathogens. 2023; 12(10).

PMID: 37887767 PMC: 10610143. DOI: 10.3390/pathogens12101251.

Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities.

Singewar K, Fladung M Funct Integr Genomics. 2023; 23(2):185.

PMID: 37243792 PMC: 10220346. DOI: 10.1007/s10142-023-01107-y.

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