Acceleration of Bone Regeneration in Critical-Size Defect Using BMP-9-Loaded NHA/ColI/MWCNTs Scaffolds Seeded with Bone Marrow Mesenchymal Stem Cells

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2019 May 22

PMID 31111065

Citations 17

Authors

Ran Zhang

Xuewen Li

Yao Liu

Xiaobo Gao

Tong Zhu

Li Lu

Affiliations

Soon will be listed here.

Abstract

Biocompatible scaffolding materials play an important role in bone tissue engineering. This study sought to develop and characterize a nano-hydroxyapatite (nHA)/collagen I (ColI)/multi-walled carbon nanotube (MWCNT) composite scaffold loaded with recombinant bone morphogenetic protein-9 (BMP-9) for bone tissue engineering by and experiments. The composite nHA/ColI/MWCNT scaffolds were fabricated at various concentrations of MWCNTs (0.5, 1, and 1.5% wt) by blending and freeze drying. The porosity, swelling rate, water absorption rate, mechanical properties, and biocompatibility of scaffolds were measured. After loading with BMP-9, bone marrow mesenchymal stem cells (BMMSCs) were seeded to evaluate their characteristics and in a critical sized defect in Sprague-Dawley rats . It was shown that the 1% MWCNT group was the most suitable for bone tissue engineering. Our results demonstrated that scaffolds loaded with BMP-9 promoted differentiation of BMMSCs into osteoblasts and induced more bone formation . To conclude, nHA/ColI/MWCNT scaffolds loaded with BMP-9 possess high biocompatibility and osteogenesis and are a good candidate for use in bone tissue engineering.

Citing Articles

Research progress of gene therapy combined with tissue engineering to promote bone regeneration.

Chu X, Xiong Y, Lu L, Wang Y, Wang J, Zeng R APL Bioeng. 2024; 8(3):031502.

PMID: 39301183 PMC: 11412735. DOI: 10.1063/5.0200551.

New Insight of Scaffold Based on Hydroxyapatite (HAp)/Bacteria's Nanocellulose (BN) for Dental Tissue Engineering.

Anitasari S, Setia Budi H, Shen Y, Arina Y Eur J Dent. 2023; 18(3):891-897.

PMID: 37995727 PMC: 11290933. DOI: 10.1055/s-0043-1776123.

PCL/Graphene Scaffolds for the Osteogenesis Process.

Anitasari S, Wu C, Shen Y Bioengineering (Basel). 2023; 10(3).

PMID: 36978696 PMC: 10044836. DOI: 10.3390/bioengineering10030305.

Functional Approaches in Promoting Vascularization and Angiogenesis in Bone Critical-Sized Defects via Delivery of Cells, Growth Factors, Drugs, and Particles.

Shineh G, Patel K, Mobaraki M, Tayebi L J Funct Biomater. 2023; 14(2).

PMID: 36826899 PMC: 9960138. DOI: 10.3390/jfb14020099.

Nano-Hydroxyapatite Composite Scaffolds Loaded with Bioactive Factors and Drugs for Bone Tissue Engineering.

Mo X, Zhang D, Liu K, Zhao X, Li X, Wang W Int J Mol Sci. 2023; 24(2).

PMID: 36674810 PMC: 9867487. DOI: 10.3390/ijms24021291.

References

Rodrigues C, Serricella P, Linhares A, Guerdes R, Borojevic R, Rossi M . Characterization of a bovine collagen-hydroxyapatite composite scaffold for bone tissue engineering. Biomaterials. 2003; 24(27):4987-97. DOI: 10.1016/s0142-9612(03)00410-1. View

Kang Q, Sun M, Cheng H, Peng Y, Montag A, Deyrup A . Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery. Gene Ther. 2004; 11(17):1312-20. DOI: 10.1038/sj.gt.3302298. View

Bottini M, Bruckner S, Nika K, Bottini N, Bellucci S, Magrini A . Multi-walled carbon nanotubes induce T lymphocyte apoptosis. Toxicol Lett. 2005; 160(2):121-6. DOI: 10.1016/j.toxlet.2005.06.020. View

Gomes M, Bossano C, Johnston C, Reis R, Mikos A . In vitro localization of bone growth factors in constructs of biodegradable scaffolds seeded with marrow stromal cells and cultured in a flow perfusion bioreactor. Tissue Eng. 2006; 12(1):177-88. DOI: 10.1089/ten.2006.12.177. View

Zanello L, Zhao B, Hu H, Haddon R . Bone cell proliferation on carbon nanotubes. Nano Lett. 2006; 6(3):562-7. DOI: 10.1021/nl051861e. View

Wahl D, Czernuszka J . Collagen-hydroxyapatite composites for hard tissue repair. Eur Cell Mater. 2006; 11:43-56. DOI: 10.22203/ecm.v011a06. View

Liu Y, Zhang S, Ma G, Zhang F, Hu R . Efficacy and safety of a live canine adenovirus-vectored rabies virus vaccine in swine. Vaccine. 2008; 26(42):5368-72. DOI: 10.1016/j.vaccine.2008.08.001. View

Narita N, Kobayashi Y, Nakamura H, Maeda K, Ishihara A, Mizoguchi T . Multiwalled carbon nanotubes specifically inhibit osteoclast differentiation and function. Nano Lett. 2009; 9(4):1406-13. DOI: 10.1021/nl8030746. View

Sehgal P, Srinivasan A . Collagen-coated microparticles in drug delivery. Expert Opin Drug Deliv. 2009; 6(7):687-95. DOI: 10.1517/17425240903025736. View

10.

Niu L, Kua H, Chua D . Bonelike apatite formation utilizing carbon nanotubes as template. Langmuir. 2009; 26(6):4069-73. DOI: 10.1021/la9034722. View

11.

Xiao Y, Gong T, Zhou S . The functionalization of multi-walled carbon nanotubes by in situ deposition of hydroxyapatite. Biomaterials. 2010; 31(19):5182-90. DOI: 10.1016/j.biomaterials.2010.03.012. View

12.

Cui H, Vashist S, Al-Rubeaan K, Luong J, Sheu F . Interfacing carbon nanotubes with living mammalian cells and cytotoxicity issues. Chem Res Toxicol. 2010; 23(7):1131-47. DOI: 10.1021/tx100050h. View

13.

Marina Ferreira A, Gentile P, Chiono V, Ciardelli G . Collagen for bone tissue regeneration. Acta Biomater. 2012; 8(9):3191-200. DOI: 10.1016/j.actbio.2012.06.014. View

14.

de Guzman R, Saul J, Ellenburg M, Merrill M, Coan H, Smith T . Bone regeneration with BMP-2 delivered from keratose scaffolds. Biomaterials. 2012; 34(6):1644-56. DOI: 10.1016/j.biomaterials.2012.11.002. View

15.

Meredith J, Jin C, Narayan R, Aggarwal R . Biomedical applications of carbon-nanotube composites. Front Biosci (Elite Ed). 2013; 5(2):610-21. DOI: 10.2741/e643. View

16.

Cheng Q, Rutledge K, Jabbarzadeh E . Carbon nanotube-poly(lactide-co-glycolide) composite scaffolds for bone tissue engineering applications. Ann Biomed Eng. 2013; 41(5):904-16. DOI: 10.1007/s10439-012-0728-8. View

17.

Boncel S, Zajac P, Koziol K . Liberation of drugs from multi-wall carbon nanotube carriers. J Control Release. 2013; 169(1-2):126-40. DOI: 10.1016/j.jconrel.2013.04.009. View

18.

Akbay E, Aydogan F . Reconstruction of isolated mandibular bone defects with non-vascularized corticocancellous bone autograft and graft viability. Auris Nasus Larynx. 2013; 41(1):56-62. DOI: 10.1016/j.anl.2013.07.002. View

19.

Steven E, Saleh W, Lebedev V, Acquah S, Laukhin V, Alamo R . Carbon nanotubes on a spider silk scaffold. Nat Commun. 2013; 4:2435. PMC: 3778718. DOI: 10.1038/ncomms3435. View

20.

He X, Liu Y, Yuan X, Lu L . Enhanced healing of rat calvarial defects with MSCs loaded on BMP-2 releasing chitosan/alginate/hydroxyapatite scaffolds. PLoS One. 2014; 9(8):e104061. PMC: 4118996. DOI: 10.1371/journal.pone.0104061. View