Genomic Landscape Analyses of Reprogrammed Cells Using Integrative and Non-integrative Methods Reveal Variable Cancer-associated Alterations

Overview

Journal Oncotarget

Specialty Oncology

Date 2019 May 21

PMID 31105870

Citations 3

Authors

Frank Griscelli

Christophe Desterke

Olivier Feraud

Dominique Divers

Noufissa Oudrhiri

Lucie Tosca

Ali G Turhan

Annelise Bennaceur-Griscelli

Affiliations

Soon will be listed here.

Abstract

Recent development of cell reprogramming technologies brought a major hope for future cell therapy applications by the use of these cells or their derivatives. For this purpose, one of the major requirements is the absence of genomic alterations generating a risk of cell transformation. Here we analyzed by microarray-based comparative genomic hybridization human iPSC generated by two non-integrative and one integrative method at pluripotent stage as well as in corresponding teratomas. We show that all iPSC lines exhibit copy number variations (CNV) of several genes deregulated in oncogenesis. These cancer-associated genomic alterations were more pronounced in virally programmed hiPSCs and their derivative teratoma as compared to those found in iPSC generated by mRNA-mediated reprogramming. Bioinformatics analysis showed the involvement of these genes in human leukemia and carcinoma. We conclude that genetic screening should become a standard procedure to ensure that hiPSCs are free from cancer-associated genomic alterations before clinical use.

Citing Articles

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