Calcitriol Exerts a Mineralization-inductive Effect Comparable to That of Vitamin C in Cultured Human Periodontium Cells

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2019 May 21

PMID 31105837

Citations 4

Authors

Hsiang-Hsi Hong

Adrienne Hong

Chun-Chieh Wang

E-Wen Huang

Cheng-Cheng Chiang

Tzung-Hai Yen

Yi-Fang Huang

Affiliations

Soon will be listed here.

Abstract

This study inspected whether calcitriol could exert a mineralization-inductive effect comparable to that of vitamin C in cultured human periodontium cells (hPDCs). The mRNA expression of the mineralization-related biomarkers core-binding factor subunit alpha-1 (Cbfa1), collagen 1 α1 (Col-I), alkaline phosphatase (ALP), osteopontin (OPN), bone sialoprotein (BSP), osteocalcin (OCN), vitamin D receptor (VDR), cementum protein 1 (CEMP-1), cementum attachment protein (CAP), interleukin 6 (IL-6), transforming growth factor-β1 (TGF-β1) and osteoprotegerin (OPG) was surveyed after incubation of hPDCs with vitamin C and calcitriol for 2 weeks. Translational expression information from ALP activity and CEMP-1 and CAP immunofluorescence assays was acquired from hPDCs at the second and third weeks. Extracellular calcifications were confirmed by von Kossa staining, Alizarin Red staining and synchrotron transmission X-ray microscopy (TXM) at the fourth and fifth weeks. It was found that both vitamin C and calcitriol not only increased mineralization-related mRNA fold-changes but also enhanced ALP activity, CEMP-1 immunofluorescence, von Kossa and Alizarin Red staining and TXM-associated calcifications. Generally, 10 M calcitriol displayed greater mineralization significance than 10 M calcitriol in the assays tested. However, vitamin C stimulated lower Cbfa1, Col-1, ALP, OPN, BSP, OCN, VDR, CEMP-1 and IL-6 mRNA fold-changes than 10 M calcitriol. Finally, TXM analysis indicated that a 10 M calcitriol treatment stimulated greater calcifications than vitamin C treatment. Therefore, the analytical results confirmed the osteo-inductive potential of vitamin C in cultured hPDCs. In contrast, 10 M calcitriol could potentially function as a substitute because it stimulates a greater mineralization effect than vitamin C or 10 M calcitriol.

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