The Sterility of Allotriploid Fish and Fertility of Female Autotriploid Fish

Overview

Journal Front Genet

Date 2019 May 21

PMID 31105746

Citations 7

Authors

Fangzhou Hu

Jingjing Fan

Qinbo Qin

Yangyang Huo

Yude Wang

Chang Wu

Qingfeng Liu

Wuhui Li

Xuan Chen

Cao Liu

Min Tao

Shi Wang

Rurong Zhao

Kaikun Luo

Shaojun Liu

Affiliations

Soon will be listed here.

Abstract

Based on the formation of an autotetraploid fish line (4nAUT, 4n = 200; F-F) derived from the distant hybridization of female red var. (RCC, 2n = 100) × male (BSB, 2n = 48), we produced autotriploid hybrids (3nAUT) by crossing females of RCC with males of 4nAUT and allotriploid hybrids (3nALT) by crossing females of (CC, 2n = 100) with males of 4nAUT. The aim of this study was to comparatively investigate the reproductive characteristics of 3nALT and 3nAUT. We investigated morphological traits, chromosomal numbers, DNA content and gonadal development in 3nAUT and 3nALT. The results indicated both 3nAUT and 3nALT possessed 150 chromosomes and were triploid hybrids. The females and males of 3nALT and males of 3nAUT had abnormal gonadal development and could not generate mature eggs or sperm, but the females of 3nAUT had normal gonadal development and generated mature eggs at 2 years old. The females of 3nAUT generated different sizes of eggs, which fertilized with haploid sperm from RCC and formed viable diploid, triploid, and tetraploid offspring. The formation of these two kinds of triploid hybrids provides an ideal model for studying the reproductive traits of triploid hybrids, which is of great value in animal genetics and reproductive biology.

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