Influence of Some Parameters on the Ability of , , and to Form Biofilms

Overview

Journal Vet World

Specialty Veterinary Medicine

Date 2019 May 16

PMID 31089318

Citations 1

Authors

Sara Lezzoum-Atek

Leila Bouayad

Taha Mossadak Hamdi

Affiliations

Soon will be listed here.

Abstract

Aim: The present study was conducted to evaluate the capacity of (L.m), (L.i), and to form biofilms on polystyrene support under different parameters by performing crystal violet (CV) staining technique.

Materials And Methods: Different suspensions were prepared with single strains and with multiple combinations of strains including two serogroups of L.m ( and ), L.i, and strains at different microbial load. Selected strains and combinations were grown in biofilms for 6 days attached to polystyrene microplates under aerobic and microaerophilic conditions. The evaluation of the power of adhesion and biofilm formation was determined by CV staining followed by the measurement of optical density at 24 h, 72 h, and 6 days incubation time with and without renewal of the culture medium.

Results: All the strains tested, presented more or less adhesion power depending on the variation of the studied parameters as well as the ability to form multispecies biofilms. Their development is more important by renewing the culture medium and increasing the initial load of bacteria. The ability to adhere and form biofilms differs from one serogroup to another within the same species. In bacterial combination, strains and species of bacteria adopt different behaviors.

Conclusion: The ability to form biofilms is a key factor in the persistence of tested strains in the environment. Our study showed that L.m, L.i, and could adhere to polystyrene and form biofilms under different conditions. More researches are necessary to understand the mechanisms of biofilm formation and the influence of different parameters in their development.

Citing Articles

Lactobacilli displacement and Candida albicans inhibition on initial adhesion assays: a probiotic analysis.

Rodriguez-Arias R, Guachi-Alvarez B, Montalvo-Vivero D, Machado A BMC Res Notes. 2022; 15(1):239.

PMID: 35799214 PMC: 9264498. DOI: 10.1186/s13104-022-06114-z.

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