Western Blot Analysis of Gonococcal Serogrouping Reagents
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Abstract
The W class antisera used in the coagglutination method of serogrouping Neisseria gonorrhoeae were analysed using sodium dodecyl sulphate polyacrylamide gel electrophoresis and western blot transfers. All were found to contain antibodies to the homologous protein II as well as antibodies to protein I group antigens. Examination of local isolates showed that some strains owed their reaction with coagglutination reagents to epitopes on their protein II not their protein I. How this may lead to difficulties when using coagglutination patterns to subdivide the W groups is discussed.
References
1.
Laemmli U
. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5.
DOI: 10.1038/227680a0.
View
2.
Johnston K, Holmes K, Gotschlich E
. The serological classification of Neisseria gonorrhoeae. I. Isolation of the outer membrane complex responsible for serotypic specificity. J Exp Med. 1976; 143(4):741-58.
PMC: 2190160.
DOI: 10.1084/jem.143.4.741.
View
3.
James J, Swanson J
. Studies on gonococcus infection. XIII. Occurrence of color/opacity colonial variants in clinical cultures. Infect Immun. 1978; 19(1):332-40.
PMC: 414084.
DOI: 10.1128/iai.19.1.332-340.1978.
View
4.
Sandstrom E, Danielsson D
. Serology of Neisseria gonorrhoeae. Classification by co-agglutination. Acta Pathol Microbiol Scand B. 1980; 88(1):27-38.
DOI: 10.1111/j.1699-0463.1980.tb02600.x.
View
5.
Danielsson D, Sandstrom E
. Serology of Neisseria gonorrhoeae. Demonstration by co-agglutination and immunoelectrophoresis of antigenic differences associated with colour/opacity colonial variants. Acta Pathol Microbiol Scand B. 1980; 88(1):39-46.
DOI: 10.1111/j.1699-0463.1980.tb02601.x.
View