Control of Cloning of Normal Human T Lymphocytes by Transferrin, Albumin and Different Lectins

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1978 Sep 1

PMID 310742

Citations 8

Authors

L Sachs

Affiliations

Soon will be listed here.

Abstract

Normal human T-lymphocytes can be induced to form colonies with a high cloning efficiency by seeding the cells directly in agar with normal human plasma and the lectins concanavalin A, phytohaemagglutinin, or pokeweed mitogen. The requirement for human plasma can be substituted, to different degrees with different lectins, by adding transferrin and albumin to foetal calf serum. This provides a useful system for identifying specific deficiencies in the requirements for normal T-lymphocyte colony formation.

Citing Articles

T lymphocyte colony-forming capacity of patients with immunodeficiency diseases: relationship of colony formation to E rosette formation and lymphocyte proliferation.

Herrod H, Valenski W Clin Exp Immunol. 1981; 45(3):562-7.

PMID: 6978208 PMC: 1537424.

Colony-forming lymphocytes in man. I. In vivo effect of hydrocortisone on phytohaemagglutinin-induced T cell colony formation.

Gupta S, Damle N Clin Exp Immunol. 1981; 45(3):557-61.

PMID: 6978207 PMC: 1537412.

The use of a T lymphocyte colony assay to measure mononuclear phagocyte function.

Gordon M, King J, Gordon-Smith E Clin Exp Immunol. 1980; 42(1):156-61.

PMID: 6970097 PMC: 1537067.

Impaired in vitro growth of PHA induced T lymphocyte colonies in hemodialyzed renal failure patients.

Ghio R, Haupt E, Pistoia V, Perata A, Minale P, Ratti M Blut. 1985; 50(3):135-40.

PMID: 3978240 DOI: 10.1007/BF00320069.

Transferrin synthesis by inducer T lymphocytes.

Lum J, Infante A, Makker D, Yang F, BOWMAN B J Clin Invest. 1986; 77(3):841-9.

PMID: 3005367 PMC: 423470. DOI: 10.1172/JCI112381.

References

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Claesson M, Rodger M, Johnson G, Whittingham S, Metcalf D . Colony formation by human T lymphocytes in agar medium. Clin Exp Immunol. 1977; 28(3):526-34. PMC: 1541018. View

Sachs L . Control of normal cell differentiation and the phenotypic reversion of malignancy in myeloid leukaemia. Nature. 1978; 274(5671):535-9. DOI: 10.1038/274535a0. View

Guilbert L, Iscove N . Partial replacement of serum by selenite, transferrin, albumin and lecithin in haemopoietic cell cultures. Nature. 1976; 263(5578):594-5. DOI: 10.1038/263594a0. View

Gerassi E, Sachs L . Regulation of the induction of colonies in vitro by normal human lymphocytes. Proc Natl Acad Sci U S A. 1976; 73(12):4546-50. PMC: 431536. DOI: 10.1073/pnas.73.12.4546. View

Bradley T, Metcalf D . The growth of mouse bone marrow cells in vitro. Aust J Exp Biol Med Sci. 1966; 44(3):287-99. DOI: 10.1038/icb.1966.28. View

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Ichikawa Y, PLUZNIK D, Sachs L . In vitro control of the development of macrophage and granulocyte colonies. Proc Natl Acad Sci U S A. 1966; 56(2):488-95. PMC: 224399. DOI: 10.1073/pnas.56.2.488. View

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PLUZNIK D, Sachs L . The induction of clones of normal mast cells by a substance from conditioned medium. Exp Cell Res. 1966; 43(3):553-63. DOI: 10.1016/0014-4827(66)90026-7. View

16.

PLUZNIK D, Sachs L . The cloning of normal "mast" cells in tissue culture. J Cell Physiol. 1965; 66(3):319-24. DOI: 10.1002/jcp.1030660309. View

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