The and Anti-hepatotoxic, Anti-hepatitis B Virus and Hepatic CYP450 Modulating Potential of

Overview

Journal Saudi Pharm J

Specialty Pharmacy

Date 2019 May 8

PMID 31061625

Citations 6

Authors

Mohammad K Parvez

Mohammed S Al-Dosari

Ahmed H Arbab

Sakina Niyazi

Affiliations

Soon will be listed here.

Abstract

In the present study we investigated the hepatotoprotective, hepatitis B virus (HBV) inhibitory and hepatic CYP450 enzyme (CYP3A4) modulatory potential of rhizome fractions. The crude ethanol-extract, including different organic and aqueous fractions were tested for cytoprotection on HepG2 cells (MTT assay), followed by evaluation in Wistar rats (serum biochemistry and lipid profile). The anti-HBV activity was tested on HepG2.2.15 cells (HBsAg and HBeAg Elisa). Of these, the n-butanol and aqueous fractions showed the most promising, dose-dependent hepatoprotection in DCFH-injured HepG2 cells. Further, in CCl-injured rats, oral administration of (100 and 200 mg/kg·bw/day) significantly normalized serum markers of healthy liver function (SGOT, SGPT, GGT, ALP and bilirubin) and lipid profile (cholesterol, HDL, LDL, VLDL, TG and MDA), including tissue NP-SH and TP levels. Compared to other fractions, the ethyl acetate, n-butanol and aqueous fractions exhibited the best inhibitory effects on viral HBsAg and HBeAg secretions in dose- and time-dependent manner. In addition, reporter gene assay (Dual-luciferase) of transfected HepG2 cells showed mild activation of nuclear PXR-mediated CYP3A4 gene by the three active fractions. Taken together, showed very promising hepatoprotective and anti-HBV potential in experimental settings In addition, this is the first report on modulation of CYP3A4 by that suggests its safe consumption in relation to drug metabolism and efficacy. Our data could therefore, provide the basis for the ethnobotanical medicinal use of in metabolic liver disorder and hepatitis B patients.

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