Multiplane Calcium Imaging Reveals Disrupted Development of Network Topology in Zebrafish Mutants

Overview

Journal eNeuro

Specialty Neurology

Date 2019 May 8

PMID 31061071

Citations 6

Authors

Sarah E W Light

James D Jontes

Affiliations

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Abstract

Functional brain networks self-assemble during development, although the molecular basis of network assembly is poorly understood. Protocadherin-19 () is a homophilic cell adhesion molecule that is linked to neurodevelopmental disorders, and influences multiple cellular and developmental events in zebrafish. Although loss of in humans and model organisms leads to functional deficits, the underlying network defects remain unknown. Here, we employ multiplane, resonant-scanning two-photon calcium imaging of developing zebrafish, and use graph theory to characterize the development of resting state functional networks in both wild-type and mutant larvae. We find that the brain networks of mutants display enhanced clustering and an altered developmental trajectory of network assembly. Our results show that functional imaging and network analysis in zebrafish larvae is an effective approach for characterizing the developmental impact of lesions in genes of clinical interest.

Citing Articles

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