Tissue-specific Signatures in Tick Cell Line MS Profiles

Overview

Journal Parasit Vectors

Publisher Biomed Central

Specialties Parasitology
Tropical Medicine

Date 2019 May 8

PMID 31060584

Citations 5

Authors

Dmitry S Loginov

Yana F Loginova

Filip Dycka

Katharina Bottinger

Pavlina Vechtova

Jan Sterba

Affiliations

Soon will be listed here.

Abstract

Background: The availability of tick in vitro cell culture systems has facilitated many aspects of tick research, including proteomics. However, certain cell lines have shown a tissue-specific response to infection. Thus, a more thorough characterization of tick cell lines is necessary. Proteomic comparative studies of various tick cell lines will contribute to more efficient application of tick cell lines as model systems for investigation of host-vector-pathogen interactions.

Results: Three cell lines obtained from a hard tick, Ixodes ricinus, and two from I. scapularis were investigated. A cell mass spectrometry approach (MALDI-TOF MS) was applied, as well as classical proteomic workflows. Using PCA, tick cell line MS profiles were grouped into three clusters comprising IRE/CTVM19 and ISE18, IRE11 and IRE/CTVM20, and ISE6 cell lines. Two other approaches confirmed the results of PCA: in-solution digestion followed by nanoLC-ESI-Q-TOF MS/MS and 2D electrophoresis. The comparison of MS spectra of the cell lines and I. ricinus tick organs revealed 29 shared peaks. Of these, five were specific for ovaries, three each for gut and salivary glands, and one for Malpighian tubules. For the first time, characteristic peaks in MS profiles of tick cell lines were assigned to proteins identified in acidic extracts of corresponding cell lines.

Conclusions: Several organ-specific MS signals were revealed in the profiles of tick cell lines.

Citing Articles

Tick Cell Culture Analysis of Growth Dynamics and Cellular Tropism of , an Endosymbiont of the Blacklegged Tick, .

Thorpe C, Wang X, Munderloh U, Kurtti T Insects. 2021; 12(11).

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First detection and genetic identification of Rickettsia infection in Rhipicephalus sanguineus (Acari: Ixodidae) ticks collected from Southern Taiwan.

Shih C, Chao L Exp Appl Acarol. 2021; 85(2-4):291-304.

PMID: 34708287 DOI: 10.1007/s10493-021-00669-5.

Molecular Detection and Genetic Identification of Infection in Ticks, an Incriminated Vector for Geographical Transmission in Taiwan.

Shih C, Yang P, Chao L Microorganisms. 2021; 9(6).

PMID: 34208514 PMC: 8233880. DOI: 10.3390/microorganisms9061309.

Interactions Between Ticks and Lyme Disease Spirochetes.

Pal U, Kitsou C, Drecktrah D, Yas O, Fikrig E Curr Issues Mol Biol. 2020; 42:113-144.

PMID: 33289683 PMC: 8045411. DOI: 10.21775/cimb.042.113.

Karyotype changes in long-term cultured tick cell lines.

Kotsarenko K, Vechtova P, Lieskovska J, Fussy Z, Cabral-de-Mello D, Rego R Sci Rep. 2020; 10(1):13443.

PMID: 32778731 PMC: 7417564. DOI: 10.1038/s41598-020-70330-5.

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