A Quick, Easy and Cost-effective Method to Develop Direct Transformants in Wheat

Overview

Journal 3 Biotech

Publisher Springer

Specialty Biotechnology

Date 2019 May 7

PMID 31058046

Citations 2

Authors

Avijit Tarafdar

Harinder Vishwakarma

S Gothandapani

Meenal Bhati

Koushik Biswas

Arul Prakash

Uttara Chaturvedi

Amolkumar U Solanke

Jasdeep Chatrath Padaria

Affiliations

Soon will be listed here.

Abstract

mediated method was used to transform Indian elite wheat genotype HD2894 with herbicide-tolerant - (5-enolpyruvylshikimate-3-phosphate synthase) gene. The apical meristems of germinated seeds were targeted for introgression of transgene. The obtained T plants were screened by spraying 1% glyphosate and only positive transformants survived. The presence of transgene was also confirmed by PCR and Southern hybridization. Using this method, 3.07% transformation rate was observed. To identify transgenic lines carrying stably integrated - gene, the transgenic populations were screened in T generation using 1% glyphosate and lines with 100% survival were considered as homozygous. No significant morpho-physiological variations were observed within the transgenic lines as compared to non-transgenic plants. The present study resulted in herbicide-tolerant transgenic wheat and provides a valuable tool for development of wheat genetic transformation.

Citing Articles

In planta transformation in wheat: an improved protocol to develop wheat transformants.

Kumar S, Vishwakarma H, Ghosh G, Singh J, Padaria J Mol Biol Rep. 2024; 51(1):407.

PMID: 38460010 DOI: 10.1007/s11033-024-09333-8.

A simple and efficient Agrobacterium-mediated in planta transformation protocol for horse gram (Macrotyloma uniflorum Lam. Verdc.).

Amal T, Karthika P, Dhandapani G, Selvakumar S, Vasanth K J Genet Eng Biotechnol. 2020; 18(1):9.

PMID: 32206908 PMC: 7090105. DOI: 10.1186/s43141-020-00023-z.

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