Antimicrobial and Antibiofilm Activity Against of (L.) Mill. Cladode Polyphenolic Extracts

Overview

Journal Antioxidants (Basel)

Date 2019 May 5

PMID 31052535

Citations 32

Authors

Federica Blando

Rossella Russo

Carmine Negro

Luigi De Bellis

Stefania Frassinetti

Affiliations

Soon will be listed here.

Abstract

Plant extracts are a rich source of natural compounds with antimicrobial properties, which are able to prevent, at some extent, the growth of foodborne pathogens. The aim of this study was to investigate the potential of polyphenolic extracts from cladodes of (L.) Mill. to inhibit the growth of some enterobacteria and the biofilm formation by . cladodes at two stages of development were analysed for total phenolic content and antioxidant activity by Oxygen Radical Absorbance Capacity (ORAC) and Trolox equivalent antioxidant capacity (TEAC) (in vitro assays) and by cellular antioxidant activity in red blood cells (CAA-RBC) (ex vivo assay). The Liquid Chromatography Time-of-Flight Mass Spectrometry (LC/MS-TOF) analysis of the polyphenolic extracts revealed high levels of piscidic acid, eucomic acid, isorhamnetin derivatives and rutin, particularly in the immature cladode extracts. cladodes extracts showed a remarkable antioxidant activity (in vitro and ex vivo), a selective inhibition of the growth of Gram-positive bacteria, and an inhibition of biofilm formation. Our results suggest and confirm that cladode extracts could be employed as functional food, due to the high polyphenolic content and antioxidant capacity, and used as natural additive for food process control and food safety.

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References

Stewart P, Costerton J . Antibiotic resistance of bacteria in biofilms. Lancet. 2001; 358(9276):135-8. DOI: 10.1016/s0140-6736(01)05321-1. View

Wu X, Beecher G, Holden J, Haytowitz D, Gebhardt S, Prior R . Lipophilic and hydrophilic antioxidant capacities of common foods in the United States. J Agric Food Chem. 2004; 52(12):4026-37. DOI: 10.1021/jf049696w. View

Wolfe K, Liu R . Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods, and dietary supplements. J Agric Food Chem. 2007; 55(22):8896-907. DOI: 10.1021/jf0715166. View

Corral-Aguayo R, Yahia E, Carrillo-Lopez A, Gonzalez-Aguilar G . Correlation between some nutritional components and the total antioxidant capacity measured with six different assays in eight horticultural crops. J Agric Food Chem. 2008; 56(22):10498-504. DOI: 10.1021/jf801983r. View

Ginestra G, Parker M, Bennett R, Robertson J, Mandalari G, Narbad A . Anatomical, chemical, and biochemical characterization of cladodes from prickly pear [Opuntia ficus-indica (L.) Mill.]. J Agric Food Chem. 2009; 57(21):10323-30. DOI: 10.1021/jf9022096. View

de Almeida C, Garbois G, Amaral L, Diniz C, Le Hyaric M . Relationship between structure and antibacterial activity of lipophilic N-acyldiamines. Biomed Pharmacother. 2009; 64(4):287-90. DOI: 10.1016/j.biopha.2009.09.013. View

Medina-Torres L, Vernon-Carter E, Gallegos-Infante J, Rocha-Guzman N, Herrera-Valencia E, Calderas F . Study of the antioxidant properties of extracts obtained from nopal cactus (Opuntia ficus-indica) cladodes after convective drying. J Sci Food Agric. 2011; 91(6):1001-5. DOI: 10.1002/jsfa.4271. View

OToole G . Microtiter dish biofilm formation assay. J Vis Exp. 2011; (47). PMC: 3182663. DOI: 10.3791/2437. View

Santos-Zea L, Gutierrez-Uribe J, Serna-Saldivar S . Comparative analyses of total phenols, antioxidant activity, and flavonol glycoside profile of cladode flours from different varieties of Opuntia spp. J Agric Food Chem. 2011; 59(13):7054-61. DOI: 10.1021/jf200944y. View

10.

Hernandez-Urbiola M, Perez-Torrero E, Rodriguez-Garcia M . Chemical analysis of nutritional content of prickly pads (Opuntia ficus indica) at varied ages in an organic harvest. Int J Environ Res Public Health. 2011; 8(5):1287-95. PMC: 3108109. DOI: 10.3390/ijerph8051287. View

11.

Daglia M . Polyphenols as antimicrobial agents. Curr Opin Biotechnol. 2011; 23(2):174-81. DOI: 10.1016/j.copbio.2011.08.007. View

12.

El-Mostafa K, El Kharrassi Y, Badreddine A, Andreoletti P, Vamecq J, El Kebbaj M . Nopal cactus (Opuntia ficus-indica) as a source of bioactive compounds for nutrition, health and disease. Molecules. 2014; 19(9):14879-901. PMC: 6270776. DOI: 10.3390/molecules190914879. View

13.

Frassinetti S, Gabriele M, Caltavuturo L, Longo V, Pucci L . Antimutagenic and antioxidant activity of a selected lectin-free common bean (Phaseolus vulgaris L.) in two cell-based models. Plant Foods Hum Nutr. 2015; 70(1):35-41. DOI: 10.1007/s11130-014-0453-6. View

14.

Duda-Chodak A, Tarko T, Satora P, Sroka P . Interaction of dietary compounds, especially polyphenols, with the intestinal microbiota: a review. Eur J Nutr. 2015; 54(3):325-41. PMC: 4365176. DOI: 10.1007/s00394-015-0852-y. View

15.

Mendez L, Flores F, Martin J, Rodriguez Rodriguez E, Diaz Romero C . Physicochemical characterization of cactus pads from Opuntia dillenii and Opuntia ficus indica. Food Chem. 2015; 188:393-8. DOI: 10.1016/j.foodchem.2015.05.011. View

16.

Albano C, Negro C, Tommasi N, Gerardi C, Mita G, Miceli A . Betalains, Phenols and Antioxidant Capacity in Cactus Pear [Opuntia ficus-indica (L.) Mill.] Fruits from Apulia (South Italy) Genotypes. Antioxidants (Basel). 2016; 4(2):269-80. PMC: 4665470. DOI: 10.3390/antiox4020269. View

17.

Sanchez E, Rivas Morales C, Castillo S, Leos-Rivas C, Garcia-Becerra L, Ortiz Martinez D . Antibacterial and Antibiofilm Activity of Methanolic Plant Extracts against Nosocomial Microorganisms. Evid Based Complement Alternat Med. 2016; 2016:1572697. PMC: 4939345. DOI: 10.1155/2016/1572697. View

18.

Doulgeraki A, Di Ciccio P, Ianieri A, Nychas G . Methicillin-resistant food-related Staphylococcus aureus: a review of current knowledge and biofilm formation for future studies and applications. Res Microbiol. 2016; 168(1):1-15. DOI: 10.1016/j.resmic.2016.08.001. View

19.

Nostro A, Guerrini A, Marino A, Tacchini M, Di Giulio M, Grandini A . In vitro activity of plant extracts against biofilm-producing food-related bacteria. Int J Food Microbiol. 2016; 238:33-39. DOI: 10.1016/j.ijfoodmicro.2016.08.024. View

20.

Bhattacharya D, Bhattacharya S, Patra M, Chakravorty S, Sarkar S, Chakraborty W . Antibacterial Activity of Polyphenolic Fraction of Kombucha Against Enteric Bacterial Pathogens. Curr Microbiol. 2016; 73(6):885-896. DOI: 10.1007/s00284-016-1136-3. View