Ectopic Expression of Causes Late-Flowering and Heterostylous Phenotypes in but Not in

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2019 May 5

PMID 31052421

Citations 11

Authors

Mei-Li Zhao

Jun Ni

Mao-Sheng Chen

Zeng-Fu Xu

Affiliations

Soon will be listed here.

Abstract

Trehalose-6-phosphate (T6P) phosphatase (TPP), a dephosphorylating enzyme, catalyzes the dephosphorylation of T6P, generating trehalose. In , we found six members of the family. Five of them , , , , and are highly expressed in female flowers or male flowers, or both, suggesting that members of the family may participate in flower development in . The wide expression of gene in various organs implied its versatile roles and thus was chosen for unraveling its biological functions during developmental process. We constructed an overexpression vector of cDNA driven by the cauliflower mosaic virus (CaMV) 35S promoter for genetic transformation. Compared with control plants, transgenic plants presented greater sucrose contents in their inflorescences and displayed late-flowering and heterostylous phenotypes. Exogenous application of sucrose to the inflorescence buds of wild-type repressed the development of the perianth and filaments, with a phenocopy of the transgenic . These results suggested that the significantly increased sucrose level in the inflorescence caused (or induced) by overexpression, was responsible for the formation of heterostylous flower phenotype. However, transgenic displayed no obvious phenotypic changes, implying that alone may not be sufficient for regulating flower development in . Our results are helpful for understanding the function of , which may regulate flower organ development by manipulating the sucrose status in plants.

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