A Functional Wound Dressing As a Potential Treatment for Cutaneous Leishmaniasis

Overview

Journal Pharmaceutics

Publisher MDPI

Date 2019 May 5

PMID 31052360

Citations 5

Authors

Francisco Alexandrino-Junior

Kattya Gyselle de Holanda E Silva

Marjorie Caroline Liberato Cavalcanti Freire

Viviane de Oliveira Freitas Lione

Elisama Azevedo Cardoso

Henrique Rodrigues Marcelino

Julieta Genre

Anselmo Gomes de Oliveira

Eryvaldo Socrates Tabosa do Egito

Affiliations

Soon will be listed here.

Abstract

Cutaneous leishmaniasis (CL) is a parasitic disease characterized by progressive skin sores. Currently, treatments for CL are limited to parenteral administration of the drug, which presents severe adverse effects and low cure rates. Therefore, this study aimed to develop poly(vinyl-alcohol) (PVA) hydrogels containing Amphotericin B (AmB) intended for topical treatment of CL. Hydrogels were evaluated in vitro for their potential to eliminate promastigote forms of spp., to prevent secondary infections, to maintain appropriate healing conditions, and to offer suitable biocompatibility. AmB was incorporated into the system in its non-crystalline state, allowing it to swell more and faster than the system without the drug. Furthermore, the AmB release profile showed a continuous and controlled behavior following Higuchi´s kinetic model. AmB-loaded-PVA-hydrogels (PVA-AmB) also showed efficient antifungal and leishmanicidal activity, no cytotoxic potential for VERO cells, microbial impermeability and water vapor permeability compatible with the healthy skin's physiological needs. Indeed, these results revealed the potential of PVA-AmB to prevent secondary infections and to maintain a favorable environment for the healing process. Hence, these results suggest that PVA-AmB could be a suitable and efficient new therapeutic approach for the topical treatment of CL.

Citing Articles

Immunoinformatics Approach to Design a Multi-Epitope Vaccine against Cutaneous Leishmaniasis.

Naz S, Aroosh A, Caner A, Atalay Sahar E, Toz S, Ozbel Y Vaccines (Basel). 2023; 11(2).

PMID: 36851219 PMC: 9967539. DOI: 10.3390/vaccines11020339.

Nanomedicine-based strategies to improve treatment of cutaneous leishmaniasis.

Goonoo N, Huet M, Chummun I, Karuri N, Badu K, Gimie F R Soc Open Sci. 2022; 9(6):220058.

PMID: 35719886 PMC: 9198523. DOI: 10.1098/rsos.220058.

Rational Design of Immunomodulatory Hydrogels for Chronic Wound Healing.

Kharaziha M, Baidya A, Annabi N Adv Mater. 2021; 33(39):e2100176.

PMID: 34251690 PMC: 8489436. DOI: 10.1002/adma.202100176.

Antifungal and Antiparasitic Drug Delivery.

Torrado J, Serrano D, Capilla J Pharmaceutics. 2020; 12(4).

PMID: 32260348 PMC: 7238172. DOI: 10.3390/pharmaceutics12040324.

Development and Characterization of a Semi-Solid Dosage Form of Meglumine Antimoniate for Topical Treatment of Cutaneous Leishmaniasis.

Berenguer D, Sosa L, Alcover M, Sessa M, Halbaut L, Guillen C Pharmaceutics. 2019; 11(11).

PMID: 31731660 PMC: 6920791. DOI: 10.3390/pharmaceutics11110613.

References

Peppas N, Bures P, Leobandung W, Ichikawa H . Hydrogels in pharmaceutical formulations. Eur J Pharm Biopharm. 2000; 50(1):27-46. DOI: 10.1016/s0939-6411(00)00090-4. View

Zoellner P, KAPP H, Smola H . Clinical performance of a hydrogel dressing in chronic wounds: a prospective observational study. J Wound Care. 2007; 16(3):133-6. DOI: 10.12968/jowc.2007.16.3.27019. View

Zhao X, Wu H, Guo B, Dong R, Qiu Y, Ma P . Antibacterial anti-oxidant electroactive injectable hydrogel as self-healing wound dressing with hemostasis and adhesiveness for cutaneous wound healing. Biomaterials. 2017; 122:34-47. DOI: 10.1016/j.biomaterials.2017.01.011. View

Bacon K, Hotez P, Kruchten S, Kamhawi S, Bottazzi M, Valenzuela J . The potential economic value of a cutaneous leishmaniasis vaccine in seven endemic countries in the Americas. Vaccine. 2012; 31(3):480-6. PMC: 3763201. DOI: 10.1016/j.vaccine.2012.11.032. View

Freire M, Alexandrino F, Marcelino H, de Souza Picciani P, de Holanda E Silva K, Genre J . Understanding Drug Release Data through Thermodynamic Analysis. Materials (Basel). 2017; 10(6). PMC: 5554032. DOI: 10.3390/ma10060651. View

Silva-Filho M, Siqueira S, Freire L, de Araujo I, de Holanda E Silva K, Medeiros A . How can micelle systems be rebuilt by a heating process?. Int J Nanomedicine. 2012; 7:141-50. PMC: 3263407. DOI: 10.2147/IJN.S25761. View

Okwor I, Uzonna J . Vaccines and vaccination strategies against human cutaneous leishmaniasis. Hum Vaccin. 2009; 5(5):291-301. DOI: 10.4161/hv.5.5.7607. View

Gu B, Sun X, Papadimitrakopoulos F, Burgess D . Seeing is believing, PLGA microsphere degradation revealed in PLGA microsphere/PVA hydrogel composites. J Control Release. 2016; 228:170-178. PMC: 4828299. DOI: 10.1016/j.jconrel.2016.03.011. View

Wan W, Campbell G, Zhang Z, Hui A, Boughner D . Optimizing the tensile properties of polyvinyl alcohol hydrogel for the construction of a bioprosthetic heart valve stent. J Biomed Mater Res. 2002; 63(6):854-61. DOI: 10.1002/jbm.10333. View

10.

Carranza-Tamayo C, Assis T, Neri A, Cupolillo E, Rabello A, Romero G . Prevalence of Leishmania infection in adult HIV/AIDS patients treated in a tertiary-level care center in Brasilia, Federal District, Brazil. Trans R Soc Trop Med Hyg. 2009; 103(7):743-8. DOI: 10.1016/j.trstmh.2009.01.014. View

11.

Ruiz H, Serrano D, Dea-Ayuela M, Bilbao-Ramos P, Bolas-Fernandez F, Torrado J . New amphotericin B-gamma cyclodextrin formulation for topical use with synergistic activity against diverse fungal species and Leishmania spp. Int J Pharm. 2014; 473(1-2):148-57. DOI: 10.1016/j.ijpharm.2014.07.004. View

12.

Escobar P, Matu S, Marques C, Croft S . Sensitivities of Leishmania species to hexadecylphosphocholine (miltefosine), ET-18-OCH(3) (edelfosine) and amphotericin B. Acta Trop. 2002; 81(2):151-7. DOI: 10.1016/s0001-706x(01)00197-8. View

13.

Peppas N . 1. Commentary on an exponential model for the analysis of drug delivery: Original research article: a simple equation for description of solute release: I II. Fickian and non-Fickian release from non-swellable devices in the form of slabs, spheres,.... J Control Release. 2014; 190:31-2. View

14.

Kim D, Chung H, Bleys J, Ghohestani R . Is paromomycin an effective and safe treatment against cutaneous leishmaniasis? A meta-analysis of 14 randomized controlled trials. PLoS Negl Trop Dis. 2009; 3(2):e381. PMC: 2637543. DOI: 10.1371/journal.pntd.0000381. View

15.

Coughlan D, Quilty F, Corrigan O . Effect of drug physicochemical properties on swelling/deswelling kinetics and pulsatile drug release from thermoresponsive poly(N-isopropylacrylamide) hydrogels. J Control Release. 2004; 98(1):97-114. DOI: 10.1016/j.jconrel.2004.04.014. View

16.

Hyon S, Cha W, Ikada Y, Kita M, Ogura Y, Honda Y . Poly(vinyl alcohol) hydrogels as soft contact lens material. J Biomater Sci Polym Ed. 1994; 5(5):397-406. DOI: 10.1163/156856294x00103. View

17.

Asilian A, Sadeghinia A, Faghihi G, Momeni A . Comparative study of the efficacy of combined cryotherapy and intralesional meglumine antimoniate (Glucantime) vs. cryotherapy and intralesional meglumine antimoniate (Glucantime) alone for the treatment of cutaneous leishmaniasis. Int J Dermatol. 2004; 43(4):281-3. DOI: 10.1111/j.1365-4632.2004.02002.x. View

18.

Sibbald R, Orsted H, Schultz G, Coutts P, Keast D . Preparing the wound bed 2003: focus on infection and inflammation. Ostomy Wound Manage. 2003; 49(11):24-51. View

19.

Solomon M, Pavlotzky F, Barzilai A, Schwartz E . Liposomal amphotericin B in comparison to sodium stibogluconate for Leishmania braziliensis cutaneous leishmaniasis in travelers. J Am Acad Dermatol. 2012; 68(2):284-9. DOI: 10.1016/j.jaad.2012.06.014. View

20.

Dou X, Li P, Schonherr H . Three-Dimensional Microstructured Poly(vinyl alcohol) Hydrogel Platform for the Controlled Formation of Multicellular Cell Spheroids. Biomacromolecules. 2017; 19(1):158-166. DOI: 10.1021/acs.biomac.7b01345. View